Sizibophelele ekunikezeni lula, ukonga ixesha kunye nokugcina imali enye-stop yenkonzo yokuthenga abathengiKulula ukusebenzisa ISELI EWONKE I-RNA ISOLATION KIT, Samkela ngokufudumeleyo amaqabane amashishini amancinci asuka kuzo zonke iindlela zokuphila, sinethemba lokuseka ishishini elinobuhlobo kunye nentsebenziswano kunye nawe kwaye uzuze injongo yokuphumelela.
Sizibophelele ekunikezeni lula, ukonga ixesha kunye nokugcina imali enye-stop yenkonzo yokuthenga abathengiKulula ukusebenzisa ISELI EWONKE I-RNA ISOLATION KIT, ngoku sijonge phambili kwintsebenziswano enkulu ngakumbi nabathengi baphesheya kweelwandle ngokusekelwe kwiinzuzo ezifanayo.Siza kusebenza ngentliziyo epheleleyo ukuphucula urhwebo kunye neenkonzo zethu.Sikwathembisa ukusebenza ngokubambisana namaqabane ezoshishino ukuphakamisa intsebenziswano yethu kwinqanaba eliphezulu kunye nokwabelana ngempumelelo kunye.Wamkelekile ngokufudumeleyo ukuba undwendwele umzi-mveliso wethu ngokunyanisekileyo.

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho). Sizibophelele ekunikezeni lula, ukugcina ixesha kunye nokugcina imali enye yenkonzo yokuthenga yomthengi kwintengiso eshushu i-China Robenidine Hydrochloride Premix Best Selling Feed Ingredient, Senze uqhagamshelwano ngokufudumeleyo kunye nobudlelwane obufudumeleyo kunye nokuseka ishishini elincinci kunye nokwamkela ngokufudumeleyo kunye nokuseka amashishini amancinci, samkela ngokufudumeleyo kunye nokuseka amashishini amancinci. kwaye uzuze injongo yokuphumelela.
Intengiso eshushu yaseChina eyongezayo, iBanga lokutyisa, ngoku sijonge phambili kwintsebenziswano enkulu ngakumbi nabathengi baphesheya kweelwandle ngokusekwe kwiinzuzo ezifanayo.Siza kusebenza ngentliziyo epheleleyo ukuphucula urhwebo kunye neenkonzo zethu.Sikwathembisa ukusebenza ngokubambisana namaqabane ezoshishino ukuphakamisa intsebenziswano yethu kwinqanaba eliphezulu kunye nokwabelana ngempumelelo kunye.Wamkelekile ngokufudumeleyo ukuba undwendwele umzi-mveliso wethu ngokunyanisekileyo.