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China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit

China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit Featured Image
  • China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit
  • China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit
  • China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit
  • China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit

Inkcazelo yeKit:

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo ukukhulula ngokukhawuleza i-DNA ye-genomic kwi-zebrafish kunye nezinye izihlunu zentlanzi zamanzi ahlambulukileyo, ama-fins omsila okanye iisampuli zeqanda leentlanzi kwiimpendulo ze-PCR, ngoko ke ifaneleka ngakumbi kuvavanyo lwemfuzo olukhulu.Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwe ngaphakathi kwe-10-30 imizuzu kwi-65 ° C.Azikho ezinye iinkqubo ezifana neprotheyini kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA ungasetyenziswa njenge template ye-PCR reaction.

I-2 × PCR Easy TM Mix (UNG) isebenzisa i-dUTP endaweni ye-dTTP ngesiseko se-2 × PCR Easy TM Mix, kwaye yongeza i-UNG enzyme (i-Uracil-N-glycosylase) enokunciphisa i-template equkethe i-dUTP ngexesha elifanayo.Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthotywa kwemveliso ye-PCR equkethe i-uracil.I-enzyme ye-UNG ayiyi kuba nayiphi na impembelelo kwi-template engenawo i-uracil, ngaloo ndlela iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa kunye nokuthintela ukuvavanywa kofuzo olukhulu.Usulelo lweemveliso zePCR lwenzekile.

I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Sineqela elisebenza kakuhle kakhulu ukujongana nemibuzo evela kubathengi.Injongo yethu "yi-100% yokwaneliseka kwabathengi ngomgangatho wemveliso yethu, ixabiso kunye nenkonzo yeqela lethu" kwaye sonwabele igama elihle phakathi kwabathengi.Ngeefektri ezininzi, sinokubonelela ngoluhlu olubanzi lwe-China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit, Ifemu yethu yakhula ngokukhawuleza ngobukhulu kunye nodumo ngenxa yokuzinikela kwayo ngokupheleleyo kwimveliso ekumgangatho ophezulu, ixabiso elikhulu lezisombululo kunye neenkonzo zabathengi ezintle.
Sineqela elisebenza kakuhle kakhulu ukujongana nemibuzo evela kubathengi.Injongo yethu "yi-100% yokwaneliseka kwabathengi ngomgangatho wemveliso yethu, ixabiso kunye nenkonzo yeqela lethu" kwaye sonwabele igama elihle phakathi kwabathengi.Ngeefektri ezininzi, sinokunika uluhlu olubanzi lweChina Extraction Kit, Nucleic Acid Extraction Kit, Inkampani yethu ihlala igxile ekuphuhliseni imarike yehlabathi.Ngoku sinabathengi abaninzi eRashiya, kumazwe aseYurophu, e-USA, kumazwe akuMbindi Mpuma nakumazwe aseAfrika.Sihlala silandela ukuba umgangatho usisiseko ngelixa inkonzo iqinisekisa ukuhlangabezana nabo bonke abathengi.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I

Buffer FP

I-Foregene Protease

I-6 × i-DNA yokulayisha i-Buffer

Icandelo II

2×PCR EasyTMUmxube(UNG)

Imiyalelo

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-1mg ye-caudal fin yentlanzi okanye amaqanda entlanzi ali-10 anokuhlolwa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ye-Anti-pollution 2 × PCR Easy TM Mix (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Iintlanzi zeZebra kunye nezinye iintlanzi zamanzi ahlambulukileyo.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

v IReagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

vReagent 6×DNA Loading Buffer ingagcinwa kwi 4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-vReagent 2 × PCR Easy TM Mix (UNG), ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (ukusebenzisa kwiintsuku ze-10).

Sineqela elisebenza kakuhle kakhulu ukujongana nemibuzo evela kubathengi.Injongo yethu "yi-100% yokwaneliseka kwabathengi ngomgangatho wemveliso yethu, ixabiso kunye nenkonzo yeqela lethu" kwaye sonwabele igama elihle phakathi kwabathengi.Ngeefektri ezininzi, sinokubonelela ngoluhlu olubanzi lwe-China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit, Ifemu yethu yakhula ngokukhawuleza ngobukhulu kunye nodumo ngenxa yokuzinikela kwayo ngokupheleleyo kwimveliso ekumgangatho ophezulu, ixabiso elikhulu lezisombululo kunye neenkonzo zabathengi ezintle.
China SupplierChina Extraction Kit, Nucleic Acid Extraction Kit, Inkampani yethu ihlala igxile ekuphuhliseni imarike yehlabathi.Ngoku sinabathengi abaninzi eRashiya, kumazwe aseYurophu, e-USA, kumazwe akuMbindi Mpuma nakumazwe aseAfrika.Sihlala silandela ukuba umgangatho usisiseko ngelixa inkonzo iqinisekisa ukuhlangabezana nabo bonke abathengi.

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