Iimveliso zethu zichongiwe ngokubanzi kwaye zithembeke ngabathengi kwaye ziya kwanelisa rhoqo ukuphuhlisa iminqweno yezoqoqosho kunye nentlalontle ye-China ye-wholesale ye-China Techstar Hipure Viral Nucleic Aicd Isolation Kit (i-magnetic beads, i-manual / i-automated extraction), Njengeqela elinamava sikwamkele ii-odolo ezenziwe ngokwezifiso.Eyona njongo iphambili yenkampani yethu kukwakha inkumbulo eyanelisayo kubo bonke abathengi, kunye nokuseka ubudlelwane beshishini bexesha elide lokuwina.
Iimveliso zethu zichongiwe ngokubanzi kwaye zithembeke ngabathengi kwaye ziya kwanelisa rhoqo ukuphuhlisa iminqweno yezoqoqosho nentlaloChina Nucleic Acid Extraction Kit, DNA / Rna Extraction Kit, Sikholelwa ekusekeni ubudlelwane bomthengi obunempilo kunye nonxibelelwano oluhle kwishishini.Intsebenziswano esondeleyo kunye nabathengi bethu isincedile ukuba senze amatyathanga obonelelo oluqinileyo kwaye sifumane inzuzo.Iimveliso zethu zisizuzele ukwamkelwa ngokubanzi kunye nokwaneliseka kwabathengi bethu abaxatyisiweyo kwihlabathi liphela.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Iimveliso zethu zichongiwe ngokubanzi kwaye zithembeke ngabathengi kwaye ziya kwanelisa rhoqo ukuphuhlisa iminqweno yezoqoqosho kunye nentlalontle ye-China ye-wholesale ye-China Techstar Hipure Viral Nucleic Aicd Isolation Kit (i-magnetic beads, i-manual / i-automated extraction), Njengeqela elinamava sikwamkele ii-odolo ezenziwe ngokwezifiso.Eyona njongo iphambili yenkampani yethu kukwakha inkumbulo eyanelisayo kubo bonke abathengi, kunye nokuseka ubudlelwane beshishini bexesha elide lokuwina.
Ivenkile yaseTshayinaChina Nucleic Acid Extraction Kit, DNA / Rna Extraction Kit, Sikholelwa ekusekeni ubudlelwane bomthengi obunempilo kunye nonxibelelwano oluhle kwishishini.Intsebenziswano esondeleyo kunye nabathengi bethu isincedile ukuba senze amatyathanga obonelelo oluqinileyo kwaye sifumane inzuzo.Iimveliso zethu zisizuzele ukwamkelwa ngokubanzi kunye nokwaneliseka kwabathengi bethu abaxatyisiweyo kwihlabathi liphela.