Ngendlela ethembekileyo yomgangatho olungileyo, irekhodi elimangalisayo kunye nenkonzo egqibeleleyo yabathengi, uthotho lwezisombululo eziveliswa lishishini lethu zithunyelwa kumazwe amaninzi nakwimimandla yeChinese Professional Ingasebenziyo Esebenzayo yokuGcinwa kweVirus Solution kunye neRnase Inhibitors, Kwaye sinokunceda ukukhangela naziphi na iimveliso zeemfuno zabathengi.Qinisekisa ukuba unikezela ngeyona Nkonzo ilungileyo, owona Mgangatho uPhezulu, uHanjiso olukhawulezayo.
Ngendlela ethembekileyo yomgangatho olungileyo, irekhodi elimangalisayo kunye nenkonzo egqibeleleyo yabathengi, uluhlu lwezisombululo eziveliswa lishishini lethu zithunyelwa kumazwe amaninzi nakwimimandlaUkuGcinwa kweNtsholongwane yaseTshayina ngeRnase Inhibitors kunye neReagent engasebenziyo yokuGcinwa kweNtsholongwane, Kuba nabani na onomdla kuyo nayiphi na impahla yethu emva kokuba ujonge uluhlu lwemveliso yethu, nceda uzive ukhululekile ngokupheleleyo ukunxibelelana nathi xa unemibuzo.Uyakwazi ukusithumelela ii-imeyile kwaye uqhagamshelane nathi ngokubonisana kwaye siya kukuphendula ngokukhawuleza njengoko sinakho.Ukuba kulula, ungayifumana idilesi yethu kwiwebhusayithi yethu kwaye uze kwishishini lethu ukuze ufumane ulwazi oluthe kratya lwezisombululo zethu ngokwakho.Besisoloko sikulungele ukwakha ubudlelwane bentsebenziswano obandisiweyo nobuzinzileyo kunye nabo nabaphi na abathengi abanokubakho kwiinkalo ezinxulumeneyo.

Iinkcukacha

5ml, 10ml, 40ml

I-PCR Hero (NgeDayi) Ikhithi yokutsalwa ibandakanya isizukulwana esitsha se-Foregene esisodwa se-Taq-Foregene Taq Hero, i-dNTPs ephuculweyo, i-MgCl2, i-reaction buffer, i-PCR reaction enhancer, i-optimizer kunye ne-stabilizer.Inkqubo ye-2 × i-PCR ye-Hero TM (i-dye) inokunyamezela okuphezulu kwi-PCR inhibitors kunenkqubo eqhelekileyo ye-PCR Mix, kwaye inokumelana ngokulula nokukhulisa i-PCR yeetemplates ezahlukeneyo eziyinkimbinkimbi.Inkqubo yokusabela ekhethekileyo kunye ne-Taq Hero ephezulu yenza ukuba i-PCR iphendule ibe nempumelelo ephezulu yokukhulisa, ukuchaneka kunye novakalelo.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukuthembeka okuphezulu: amaxesha ama-6 kune-enzyme yeTaq eqhelekileyo;

■ Isantya sokukhulisa ngokukhawuleza : isantya sokukhulisa yi-5-10sec/kb, ephindwe ka-6-12 kune-enzyme ye-Taq eqhelekileyo;

■ Ukuguquguquka okuthe kratya kwetemplate: Inokukhulisa ngokufanelekileyo iitemplate ezahlukeneyo ze-DNA ezintsonkothileyo eziphezuluUmxholo weGC;

■ Ubuchule obuphezulu bokwandisa: imijikelo esezantsi yokwandisa kune-enzyme yeTaq eqhelekileyo;

■ Ukunyamezela kokusingqongileyo kunamandla: ibekwe kwi-37 ° C ngeveki, igcina umsebenzi ongaphezulu kwe-90%;

■ Inomsebenzi we-5′ →3′ DNA polymerase kunye no-5′→3′ umsebenzi we-exonuclease, ngaphandle kwe-3′ →5′ umsebenzi we-exonuclease.

■ Ikiti ixutywe kwangaphambili kunye ne-Electrophoresis Loading Buffer, emva kokuba i-PCR igqityiwe, isampuli inokulayishwa ngokuthe ngqo kwi-electrophoresis;ukusebenza kulula kwaye kulula.

Isicelo seKit

Ukwandiswa kwePCR yamaqhekeza eDNA, ukuleyibhelishwa kweDNA, ulandelelwano lweDNA, PCR kunye nomsila

Ugcino kunye nobomi beShelf

Inokugcinwa ixesha elide kwi -20 ° C;ukusetyenziswa rhoqo, inokugcinwa kwi-4 ° C ixesha elifutshane.Ngendlela ethembekileyo yomgangatho omhle, irekhodi elimangalisayo kunye nenkonzo egqibeleleyo yabathengi, uluhlu lwezisombululo eziveliswa lishishini lethu zithunyelwa kumazwe amaninzi kunye nemimandla ye-Chinese Professional Inactivated Efficient Virus Preservation Solution kunye ne-Rnase Inhibitors, Kwaye sinokukunceda ukukhangela nayiphi na imveliso yeemfuno zabathengi.Qinisekisa ukuba unikezela ngeyona Nkonzo ilungileyo, owona Mgangatho uPhezulu, uHanjiso olukhawulezayo.
Ubungcali baseTshayinaUkuGcinwa kweNtsholongwane yaseTshayina ngeRnase Inhibitors kunye neReagent engasebenziyo yokuGcinwa kweNtsholongwane, Kuba nabani na onomdla kuyo nayiphi na impahla yethu emva kokuba ujonge uluhlu lwemveliso yethu, nceda uzive ukhululekile ngokupheleleyo ukunxibelelana nathi xa unemibuzo.Uyakwazi ukusithumelela ii-imeyile kwaye uqhagamshelane nathi ngokubonisana kwaye siya kukuphendula ngokukhawuleza njengoko sinakho.Ukuba kulula, ungayifumana idilesi yethu kwiwebhusayithi yethu kwaye uze kwishishini lethu ukuze ufumane ulwazi oluthe kratya lwezisombululo zethu ngokwakho.Besisoloko sikulungele ukwakha ubudlelwane bentsebenziswano obandisiweyo nobuzinzileyo kunye nabo nabaphi na abathengi abanokubakho kwiinkalo ezinxulumeneyo.