Ngolawulo lwethu olugqwesileyo, amandla obuchule obuqinileyo kunye nendlela engqongqo yokulawula egqwesileyo, siqhuba sinikezela abathengi bethu umgangatho olungileyo olungileyo, iindleko ezifanelekileyo kunye neenkampani ezinkulu.Sijonge ekuthatheni ingqalelo njengelinye lawona maqabane anoxanduva kunye nokufumana uyolo lwakho kwi-Supply OEM Virus DNA kunye ne-Rna Isolation Extraction Kit, sijonge ngokunyanisekileyo ukuseka ubudlelwane obuhle bentsebenziswano kunye nabathengi abavela ekhaya naphesheya ukudala ikamva eliqaqambileyo kunye.
Ngolawulo lwethu olugqwesileyo, amandla obuchule obuqinileyo kunye nendlela engqongqo yokulawula egqwesileyo, siqhuba sinikezela abathengi bethu umgangatho olungileyo olungileyo, iindleko ezifanelekileyo kunye neenkampani ezinkulu.Sijonge ekubeni sithathelwe ingqalelo njengelinye lawona maqabane anoxanduva kwaye sifumane ulonwabo lwakhoI-China Nucleic Acid Reagent kunye ne-DNA / Rna Extraction Kit, Ngoku sineminyaka engaphezu kwe-10 yamava emveliso kunye neshishini lokuthumela ngaphandle.Sihlala siphuhlisa kwaye siyila iintlobo zentengiso yenoveli ukuhlangabezana neemfuno zentengiso kwaye sincede iindwendwe ngokuqhubekayo ngokuhlaziya iimveliso zethu.Sibe ngumenzi okhethekileyo kunye nomthengisi e-China.Naphi na apho ukhoyo, qiniseka ukuba usijoyina, kwaye kunye siya kubumba ikamva eliqaqambileyo kwishishini lakho!
Izikhokelo zoMyalelo:

Ngolawulo lwethu olugqwesileyo, amandla obuchule obuqinileyo kunye nendlela engqongqo yokulawula egqwesileyo, siqhuba sinikezela abathengi bethu umgangatho olungileyo olungileyo, iindleko ezifanelekileyo kunye neenkampani ezinkulu.Sijonge ekuthatheni ingqalelo njengelinye lawona maqabane anoxanduva kunye nokufumana uyolo lwakho kwi-Supply OEM Virus DNA kunye ne-Rna Isolation Extraction Kit, sijonge ngokunyanisekileyo ukuseka ubudlelwane obuhle bentsebenziswano kunye nabathengi abavela ekhaya naphesheya ukudala ikamva eliqaqambileyo kunye.
Ukubonelela nge-OEMI-China Nucleic Acid Reagent kunye ne-DNA / Rna Extraction Kit, Ngoku sineminyaka engaphezu kwe-10 yamava emveliso kunye neshishini lokuthumela ngaphandle.Sihlala siphuhlisa kwaye siyila iintlobo zentengiso yenoveli ukuhlangabezana neemfuno zentengiso kwaye sincede iindwendwe ngokuqhubekayo ngokuhlaziya iimveliso zethu.Sibe ngumenzi okhethekileyo kunye nomthengisi e-China.Naphi na apho ukhoyo, qiniseka ukuba usijoyina, kwaye kunye siya kubumba ikamva eliqaqambileyo kwishishini lakho!

Isikhokelo sokuHlalutya ingxaki

Oku kulandelayo kuhlalutyo lweengxaki ezinokujamelana nazo ekutsalweni kwe-viral DNA / RNA, ngethemba lokuba luncedo kwiimvavanyo zakho.Ukongeza, kwezinye iingxaki zovavanyo okanye zobugcisa ngaphandle kwemiyalelo yokusebenza kunye nohlalutyo lwengxaki, sinikezele ngenkxaso yobugcisa ukukunceda.Ukuba unazo naziphi na iimfuno, nceda uqhagamshelane nathi: 028-83360257 okanye i-imeyile:

Tech@foregene.com.

Akukho kukhutshwa kwe-nucleic acid okanye isivuno esiphantsi se-nucleic acid

Ngokuqhelekileyo kukho izinto ezininzi ezichaphazela ukusebenza kakuhle kokubuyisela, njenge: isampuli ye-nucleic acid umxholo, indlela yokusebenza, umthamo we-lution, njl.

Uhlalutyo lwezizathu eziqhelekileyo:

1. Ukuhlamba iqhwa okanye ukushisa okuphantsi (4 ° C) centrifugation kwenziwa ngexesha lenkqubo.

Ingcebiso: Sebenza kwiqondo lobushushu begumbi (15-25°C) kuyo yonke le nkqubo, musa ukuhlamba umkhenkce kunye ne-centrifugation yobushushu obuphantsi.

2. Isampuli igcinwe ngokungafanelekanga okanye isampuli igcinwe ixesha elide.

Isincomo: Gcina iisampuli kwi-80 ° C kwaye ugweme ukukhenkceza ngokuphindaphindiweyo kunye nokunyibilika;zama ukusebenzisa iisampulu ezisanda kuqokelelwa ukutsalwa kwe-nucleic acid.

3. I-lysis yesampuli eyaneleyo.

Isincomo: Nceda uqinisekise ukuba isampula kunye nesisombululo sokusebenza se-lysis sixutywe ngokucokisekileyo kwaye sifakwe kwindawo yokushisa (15-25 ° C) imizuzu eyi-10.

4. Ukongezwa okungalunganga kwe-eluent.

Ingcebiso: Qinisekisa ukuba i-ddH2O ye-RNase-Free yongezwa phakathi kwe-membrane yekholomu yokucoca, kwaye ungayilahli kwiringi yekholomu yokucoca.

5. Umthamo ochanekileyo we-ethanol ngokupheleleyo awuzange wongezwe kwi-Buffer RW2.

Ingcebiso: Nceda ulandele imiyalelo, yongeza umthamo ochanekileyo we-ethanol epheleleyo kwi-Buffer RW2 kwaye udibanise kakuhle phambi kokusebenzisa ikhithi.

6. Umthamo wesampuli engafanelekanga.

Iingcebiso: 200µl yesampulu isetyenzwa kwi-500µl nganye ye-Buffer DRL.Ukusetyenzwa kweesampulu ezigqithisileyo kuya kubangela isivuno esisezantsi se-nucleic acid extraction.

7. Umthamo wesandi esingafanelekanga okanye i-elution engaphelelanga.

Isincomo: Umthamo ocacileyo wekholamu yokucoca yi-30-50μl;ukuba i-elution effect ayinelisi, kuyacetyiswa ukuba kwandiswe ixesha kwiqondo lobushushu legumbi emva kokongeza i-ddH2O ye-RNase-Free preheated, efana ne-5-10min.

8. I-Ethanol ihlala kwikholamu emva kokuhlamba nge-Buffer RW2.

Isiphakamiso: Ukuba i-ethanol ihlala emva kwe-centrifugation kunye ne-Buffer RW2 imizuzu ye-2, ikholamu inokubekwa kwindawo yokushisa kwemizuzu emi-5 emva kwe-centrifugation ukususa ngokupheleleyo i-ethanol eseleyo.

I-nucleic acid ecocekileyo iyancipha

Umgangatho we-nucleic acid ecocekileyo inxulumene nokugcinwa kwesampuli, ukungcoliswa kwe-RNase, ukusebenza kunye nezinye izinto.Uhlalutyo lwezizathu eziqhelekileyo:

1. Iisampuli eziqokelelweyo azigcinwanga ngexesha.

Isiphakamiso: Ukuba isampuli ayisetyenziswanga ngexesha emva kokuqokelela, nceda uyigcine kwi -80 ° C kwiqondo lokushisa eliphantsi ngokukhawuleza.Ukukhutshwa kwe-RNA, zama ukusebenzisa iisampulu ezisanda kuqokelelwa.

2. Qokelela iisampuli kwaye umkhenkce kwaye unyibilike ngokuphindaphindiweyo.

Isiphakamiso: Gwema ukukhenkceza kunye nokunyibilika (kungekho ngaphezu kweyodwa) ngexesha lokuqokelela kunye nokugcinwa kweesampuli, ngaphandle koko isivuno se-nucleic acid siya kuncitshiswa.

3. I-RNase yaziswa kwigumbi lokusebenza okanye iiglavu ezilahlwayo, iimaski, njl.njl. azinxitywa.

Ingcebiso: Imifuniselo yokutsalwa kwe-RNA yenziwa ngcono kwigumbi lokusebenza elahlukileyo le-RNA, kwaye itafile yelabhoratri kufuneka icocwe phambi kovavanyo.

Nxiba iiglavu ezilahlwayo kunye nemaski ngexesha lovavanyo ukunqanda ukuthotywa kwe-RNA okubangelwa kukwaziswa kwe-RNase kowona mgangatho mkhulu.

4. I-reagent ingcolisekile nge-RNase ngexesha lokusetyenziswa.

Ingcebiso: Faka endaweni entsha yeViral DNA/RNA Isolation Kit ukulungiselela imifuniselo enxulumeneyo.

5. Iityhubhu ze-centrifuge kunye neengcebiso ze-pipette ezisetyenziselwa ukuguqulwa kwe-RNA zingcolile nge-RNase.

Ingcebiso: Qinisekisa ukuba iityhubhu ze-centrifuge, iingcebiso ze-pipette, iipayipi, njl njl. ezisetyenziselwa ukukhutshwa kwe-RNA zonke zi-RNase-Free.

I-asidi ye-nucleic ecocekileyo ichaphazela iimvavanyo ezisezantsi

I-DNA kunye ne-RNA ehlanjululwe yikholomu yokucoca, ukuba i-ion yetyuwa kunye neprotheyini iphezulu kakhulu, iya kuchaphazela iimvavanyo ezisezantsi, ezifana: ukukhulisa i-PCR, ukuguqulelwa kwe-reverse transcription, njl.

1. I-DNA ekhutshiweyo kunye ne-RNA zineeyoni zetyuwa ezishiyekileyo.

Isiphakamiso: Qinisekisa ukuba umthamo ochanekileyo we-ethanol epheleleyo yongezwa kwi-Buffer RW2, kwaye uhlambe ikholamu yokucoca kabini kwisantya se-centrifugation esichazwe kwimiyalelo yokusebenza;Yenza i-centrifugation ukunciphisa ukungcoliseka kwe-ion yetyuwa.

2. I-DNA ekhutshiwe kunye ne-RNA zineentsalela ze-ethanol.

Isiphakamiso: Emva kokuqinisekisa ukuhlamba nge-Buffer RW2, yenza i-tube centrifugation engenanto kwisantya se-centrifugation kwimiyalelo yokusebenza;ukuba kusekho intsalela ye-ethanol, unako ukwenza i-centrifuge ityhubhu engenanto kwaye uyibeke kwindawo yokushisa kwemizuzu emi-5 ukususa intsalela ye-ethanol ukuya kwinqanaba elikhulu.

Izikhokelo zoMyalelo:

I-Viral DNA & RNA Isolation Kit Manual Manual