Ukuqina kwethu kubambelela kumgaqo osisiseko othi "Umgangatho unokuba bubomi bombutho wakho, kwaye igama linokuba ngumphefumlo walo"khupha i-RNA iyonke, Enkosi ngokuthatha ixesha lakho elifanelekileyo lokuya kuthi kwaye uhlale ukuze ube nentsebenziswano entle kunye nawe.
Ukuqina kwethu kubambelela kumgaqo osisiseko othi "Umgangatho unokuba bubomi bombutho wakho, kwaye igama linokuba ngumphefumlo walo"khupha i-RNA iyonke, Inkampani yethu ithembisa: amaxabiso afanelekileyo, ixesha elifutshane lokuvelisa kunye nenkonzo eyanelisayo emva kokuthengisa, siyakwamkela ukuba undwendwele umzi-mveliso wethu nangaliphi na ixesha ofuna.Ndinqwenela ukuba sineshishini elimnandi nelide kunye !!!

Iinkcukacha

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Inkampani yethu ibambelele kumgaqo osisiseko othi "Umgangatho unokuba bubomi bombutho wakho, kwaye igama linokuba ngumphefumlo walo" ngeXabiso eliPhezulu kwi-China Athletic kunye ne-Aplikheshini yezoNyango yokuPhilisa iBone Skin kunye ne-Muscle Tissue L-Glutamine Amino Acid, Enkosi ngokuthatha ixesha lakho elifanelekileyo lokuya kuthi kwaye uhlale ulindele ukuba nentsebenziswano emnandi kunye nawe.
Ixabiso Elihle kakhulu kwi-China Additive Food, Athletic, Inkampani yethu ithembisa: amaxabiso afanelekileyo, ixesha elifutshane lokuvelisa kunye nenkonzo eyanelisayo emva kokuthengisa, siyakwamkela ukuba undwendwele umzi-mveliso wethu nangaliphi na ixesha olifunayo.Ndinqwenela ukuba sineshishini elimnandi nelide kunye !!!