Ibambelela kumgaqo wakho othi "Inyanisekile, ikhuthele, inomdla, ivelisa izinto ezintsha" ukuvelisa izisombululo ezitsha rhoqo.Ijonga abathengi, impumelelo njengempumelelo yayo.Masiphuhlise ikamva elinempumelelo kwisandla sokuFika okuNtsha kweTshayina iLongyu Ukubonelela ngoMgangatho othembekileyo woMgangatho oPhezulu weRNA, Siyabamkela bonke abathengi kunye nabahlobo ukuba baqhagamshelane nathi ukuze bafumane izibonelelo ezifanayo.Ndiyathemba ukwenza ishishini elongezelelweyo nawe.
Ibambelela kumgaqo wakho othi "Inyanisekile, ikhuthele, inomdla, ivelisa izinto ezintsha" ukuvelisa izisombululo ezitsha rhoqo.Ijonga abathengi, impumelelo njengempumelelo yayo.Masiphuhlise ikamva elinempumelelo ngesandla sibambeneChina Passion High RNA umgangatho, Ukutsalwa kwesinyibilikisi, Injongo yethu kukunceda abathengi baqonde iinjongo zabo.Senza iinzame ezinkulu zokufezekisa le meko yokuphumelela kwaye samkele ngokunyanisekileyo ukuba usijoyine.Ngelizwi, xa usikhetha, ukhetha ubomi obugqibeleleyo.Wamkelekile ukutyelela umzi-mveliso wethu kwaye wamkele iodolo yakho!Ngemibuzo engaphezulu, nceda ungathandabuzi ukuqhagamshelana nathi.

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Ibambelela kumgaqo wakho othi "Inyanisekile, ikhuthele, inomdla, ivelisa izinto ezintsha" ukuvelisa izisombululo ezitsha rhoqo.Ijonga abathengi, impumelelo njengempumelelo yayo.Masiphuhlise ikamva elinempumelelo kwisandla sokuFika okuNtsha kweChina iLongyu Sibonelele ngeSiqhamo seSiqhamo esithembekileyo soMgangatho, Siyabamkela bonke abathengi kunye nabahlobo ukuba baqhagamshelane nathi ukuze bafumane izibonelelo ezifanayo.Ndiyathemba ukwenza ishishini elongezelelweyo nawe.
Ukufika okutsha kweChina Passion Fruit Extract,Ukutsalwa kwesinyibilikisi, Injongo yethu kukunceda abathengi baqonde iinjongo zabo.Senza iinzame ezinkulu zokufezekisa le meko yokuphumelela kwaye samkele ngokunyanisekileyo ukuba usijoyine.Ngelizwi, xa usikhetha, ukhetha ubomi obugqibeleleyo.Wamkelekile ukutyelela umzi-mveliso wethu kwaye wamkele iodolo yakho!Ngemibuzo engaphezulu, nceda ungathandabuzi ukuqhagamshelana nathi.