Ukukwazi ukwanelisa iimfuno zomxhasi ngokufanelekileyo, yonke imisebenzi yethu yenziwa ngokungqongqo ngokuhambelana nesaci sethu esithi “Umgangatho oPhezulu, ithegi yeXabiso eliKhuphisanayo, iNkonzo eKhawulezayo” yeFactory Cheap Hot China Isitokhwe esikhulu seNdalo yeSitsalo seMyricetin 529-44-2, Ubukho obuqhubekayo bomgangatho obalulekileyo wentengiso ngokudityaniswa nenkxaso egqwesileyo ethe kratya kwintengiso yehlabathi jikelele kunye nokuqinisekisa kwangaphambili okugqwesileyo kwimarike.
Ukukwazi ukwanelisa iimfuno zomxhasi ngokufanelekileyo, yonke imisebenzi yethu yenziwa ngokungqongqo ngokuhambelana nesaci sethu esithi “Umgangatho oPhezulu oPhezulu, ithegi yeXabiso eliKhuphisanayo, iNkonzo eKhawulezayo” ye.529-44-2, eTshayina Myricetin, Ngenjongo "yokukhuphisana nomgangatho olungileyo kwaye uphuhlise ngokuyila" kunye nomgaqo wenkonzo "yokuthatha imfuno yabathengi njengento yokuqhelanisa", siza kubonisa ngokunyanisekileyo iimveliso ezifanelekileyo kunye nenkonzo elungileyo kubathengi basekhaya nabamazwe ngamazwe.

Iinkcukacha

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho). Ukuze ukwazi ukwanelisa ngokufanelekileyo iimfuno zomxhasi, yonke imisebenzi yethu yenziwa ngokungqongqo ngokuhambelana nesiqubulo sethu esithi "Umgangatho oPhezulu, iThegi yexabiso eliKhuphisanayo, iNkonzo eKhawulezayo" yeFactory Eshibhile eHotyo yaseTshayina, i-Large9 yesitokhwe seNdalo ye-5 ebalulekileyo ye-5 i-ava. imveliso edityanisiweyo nenkxaso yethu ebalaseleyo yaphambi nasemva kwentengiso iqinisekisa ukhuphiswano olumandla kwimakethi ekhulayo yehlabathi.
Factory Cheap Hot China Myricetin, 529-44-2, Ngenjongo "ukukhuphisana nomgangatho olungileyo kwaye uphuhlise ngokuyila" kunye nomgaqo wenkonzo "thatha imfuno yabathengi 'njengokuqhelanisa", siya ngokunyanisekileyo ukubonisa iimveliso ezifanelekileyo kunye nenkonzo elungileyo kubathengi basekhaya nakwamanye amazwe.