Injongo yethu iya kuba kukubonelela ngezinto ezisemgangathweni kumaxabiso akhuphisanayo, kunye nenkonzo ephezulu kubathengi kwihlabathi liphela.Siyi-ISO9001, CE, kunye ne-GS eqinisekisiweyo kwaye sithobela ngokungqongqo iinkcukacha zabo zomgangatho we-High Performance China Indlela ye-Rna Nucleic Acid Extraction Kit kunye neKit yokucoca, Ukuze ufumane inkcazelo engakumbi, nceda uqhagamshelane nathi ngokukhawuleza!
Injongo yethu iya kuba kukubonelela ngezinto ezisemgangathweni kumaxabiso akhuphisanayo, kunye nenkonzo ephezulu kubathengi kwihlabathi liphela.Siyi-ISO9001, CE, kunye ne-GS eqinisekisiweyo kwaye sibambelela ngokungqongqo kwiinkcukacha zabo zomgangathoChina Nucleic Acid Extraction, Indlela ye-Magnetic Bead, Bayimodeli ehlala ixesha elide kwaye bakhuthaza ngempumelelo kwihlabathi liphela.Akukho naphantsi kweemeko ezilahlekileyo imisebenzi emikhulu ngexesha elikhawulezayo, kuyafuneka kwimeko yakho yomgangatho olungileyo.Ikhokelwa ngumgaqo “woBuchule, uBuchule, iManyano kunye noTshintsho.Inkampani yenza iinzame ezimanyumnyezi ukwandisa urhwebo lwayo lwamazwe ngamazwe, ukunyusa inzuzo yenkampani kunye nokunyusa isikali sayo sokuthumela ngaphandle.Siqinisekile ukuba siza kuba nethemba eliqaqambileyo kwaye siza kusasazwa kwihlabathi liphela kwiminyaka ezayo.

Inkcazo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) .Injongo yethu iya kuba kukunika izinto ezilungileyo ezisemgangathweni kumazinga okukhuphisana, kunye nenkonzo ye-top-notch kubathengi emhlabeni jikelele.Siyi-ISO9001, CE, kunye ne-GS eqinisekisiweyo kwaye sithobela ngokungqongqo iinkcukacha zabo zomgangatho we-High Performance China Indlela ye-Rna Nucleic Acid Extraction Kit kunye neKit yokucoca, Ukuze ufumane inkcazelo engakumbi, nceda uqhagamshelane nathi ngokukhawuleza!
Ukwenza okuphezuluChina Nucleic Acid Extraction, Indlela ye-Magnetic Bead, Bayimodeli ehlala ixesha elide kwaye bakhuthaza ngempumelelo kwihlabathi liphela.Akukho naphantsi kweemeko ezilahlekileyo imisebenzi emikhulu ngexesha elikhawulezayo, kuyafuneka kwimeko yakho yomgangatho olungileyo.Ikhokelwa ngumgaqo “woBuchule, uBuchule, iManyano kunye noTshintsho.Inkampani yenza iinzame ezimanyumnyezi ukwandisa urhwebo lwayo lwamazwe ngamazwe, ukunyusa inzuzo yenkampani kunye nokunyusa isikali sayo sokuthumela ngaphandle.Siqinisekile ukuba siza kuba nethemba eliqaqambileyo kwaye siza kusasazwa kwihlabathi liphela kwiminyaka ezayo.