Ishishini lethu kususela ekuqalweni kwalo, lihlala lisijonga isisombululo esibalaseleyo njengobomi beshishini, lihlala liqinisa itekhnoloji yemveliso, liphucula umgangatho ophezulu wemveliso kwaye liqinise ngokuqhubekayo intlangano yolawulo oluphezulu oluphezulu, ngokuhambelana ngqongqo kusetyenziswa umgangatho wesizwe we-ISO 9001:2000 ngexabiso elicatshulwe eChina CE Yamkelwe i-Nucleic Acid 64 Well Plate DNA Rna Extraction Isolation Kit, Wamkelekile ukubamba i-PC yethu ye-Diagnostic Isolation Kit. siza kukunika i-surprice ye-Qulity kunye neXabiso.
Ishishini lethu kususela ekuqalweni kwalo, lihlala lisithatha isisombululo njengobomi beshishini, lihlala liqinisa itekhnoloji yemveliso, liphucula umgangatho wemveliso kwaye lihlala liqinisa intlangano yolawulo olukumgangatho ophezulu, ngokuhambelana ngqongqo kusetyenziswa umgangatho wesizwe we-ISO 9001:2000China Viral Nucleic Acid Isolation, Ixesha langempela le-PCR Diagnostic, Sithatha imilinganiselo ngazo naziphi na iindleko ukuze sifezekise eyona sixhobo sihlaziyiweyo kunye neendlela.Ukupakishwa kwebrendi etyunjiweyo lolunye uphawu olwahlulayo.Iimveliso zokuqinisekisa iminyaka yenkonzo engenazinkathazo zitsale abathengi abaninzi.Izisombululo zifumaneka kuyilo oluphuculweyo kunye ne-assortment etyebileyo, zenziwe ngokwenzululwazi ngezinto ezikrwada kuphela.Ifumaneka ngokulula kwiindidi ezahlukeneyo zoyilo kunye neenkcukacha zokhetho lwakho.Iintlobo zamva nje zingcono kakhulu kunezo zandulelayo kwaye zithandwa kakhulu ngamathemba amaninzi.

Inkcazo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ inyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol(ukhetho).Ishishini lethu ukusukela ekusekweni kwalo, lihlala lisijonga isisombululo esibalaseleyo njengobomi beshishini, lihlala liqinisa iteknoloji yemveliso, liphucula umgangatho ophezulu wemveliso kwaye lihlala liqinisa ulawulo oluphezulu lwentlangano, ngokungqinelana ngokungqongqo kusetyenziswa umgangatho wesizwe we-ISO200001 we-Account ye-Account ye-Account 4 ye-Account ye-Account 4 ye-Account ye-Account 4 ye-Account ye-Ac. I-Plate DNA Rna Extraction Isolation Kit ye-PCR Virus Rapid Diagnostic, Wamkelekile ukuba usibambe kuye nabani na onomdla kwiimveliso zethu, siza kukunika i-surprice ye-Qulity kunye neXabiso.
Ixabiso elicatshulweyo leChina Viral Nucleic Acid Isolation, Ixesha langempela le-PCR Diagnostic, Sithatha imilinganiselo ngazo naziphi na iindleko ukuze sifezekise eyona sixhobo sihlaziyiweyo kunye neendlela.Ukupakishwa kwebrendi etyunjiweyo lolunye uphawu olwahlulayo.Iimveliso zokuqinisekisa iminyaka yenkonzo engenazinkathazo zitsale abathengi abaninzi.Izisombululo zifumaneka kuyilo oluphuculweyo kunye ne-assortment etyebileyo, zenziwe ngokwenzululwazi ngezinto ezikrwada kuphela.Ifumaneka ngokulula kwiindidi ezahlukeneyo zoyilo kunye neenkcukacha zokhetho lwakho.Iintlobo zamva nje zingcono kakhulu kunezo zandulelayo kwaye zithandwa kakhulu ngamathemba amaninzi.