Njengendlela yokukubonisa ngokulula kunye nokwandisa ishishini lethu, sikwanabo nabahloli kwi-QC Staff kwaye sikuqinisekisa ngeyona nkampani yethu ilungileyo kunye nemveliso yesampulu yasimahla yeFactory.China Viral Nucleic Acid (DNA/RNA) Miniprep Kit, Virus Rna Extraction Magnetic Beads V4002, 200 Preps, Sijonge phambili ekusekeni imibutho yamashishini exesha elide kunye nawe.Amagqabaza kunye neengcebiso zakho zixatyiswe kakhulu.
Njengendlela yokukubonisa ngokulula kunye nokwandisa ishishini lethu, sikwanabo nabahloli kwi-QC Staff kwaye sikuqinisekisa ngeyona nkampani yethu ilungileyo kunye nemveliso(DNA/RNA) Miniprep Kit, China Viral Nucleic Acid, Sinikezela ngeenkonzo ezifanelekileyo, impendulo ekhawulezileyo, ukuhanjiswa kwangexesha, umgangatho obalaseleyo kunye nexabiso elingcono kubathengi bethu.Ukwaneliseka kunye nekhredithi elungileyo kuye wonke umthengi yinto ephambili yethu.Sijolisa kuzo zonke iinkcukacha zokucwangciswa kweodolo kubathengi bade bafumane iimveliso ezikhuselekileyo nezivakalayo ezinenkonzo yolungiselelo kunye neendleko zoqoqosho.Kuxhomekeke koku, iimveliso zethu zithengiswa kakuhle kakhulu kumazwe ase-Afrika, kuMbindi-Mpuma kunye nakuMzantsi-mpuma Asia.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokufaka i-2-hydroxy-1-ethanethiol (ukhetho) .Njengendlela yokukubonisa ngokukhululeka kunye nokwandisa ishishini lethu, sikwanabo nabahloli kwi-QC Staff kwaye siqinisekisa inkampani yethu egqwesileyo kunye nemveliso yeFactory Free isampuli ye-China Viral Nucleic Acid(DNA/RNA) Miniprep Kit, Virus Rna Extraction Magnetic Beads V4002, 200 Preps, Sijonge phambili ekusekeni imibutho yamashishini exesha elide kunye nawe.Amagqabaza kunye neengcebiso zakho zixatyiswe kakhulu.
Isampulu yasimahla yeFactory ye-China Viral Nucleic Acid, (DNA/RNA) Miniprep Kit, Sinikezela ngenkonzo efanelekileyo, impendulo ekhawulezileyo, ukuhanjiswa kwangethuba, umgangatho ogqwesileyo kunye nexabiso elihle kubathengi bethu.Ukwaneliseka kunye nekhredithi elungileyo kuye wonke umthengi yinto ephambili yethu.Sijolisa kuzo zonke iinkcukacha zokucwangciswa kweodolo kubathengi bade bafumane iimveliso ezikhuselekileyo nezivakalayo ezinenkonzo yolungiselelo kunye neendleko zoqoqosho.Kuxhomekeke koku, iimveliso zethu zithengiswa kakuhle kakhulu kumazwe ase-Afrika, kuMbindi-Mpuma kunye nakuMzantsi-mpuma Asia.