Izinto zethu zichongwa ngokuqhelekileyo kwaye zithenjwa ngabathengi kwaye zinokuzalisekisa ngokuqhubekayo ukutshintshela iimfuno zezoqoqosho kunye nezentlalo zeFactory yokwenza iLula kunye neXesha eliKhawulezayo le-PCR Kit kunye ne-High Rox + Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Ukususela ekubeni indawo yokuvelisa yasekwa, ngoku sizibophelele kwinkqubela phambili yeemveliso ezintsha.Ngelixa sisebenzisa isantya sentlalo kunye nezoqoqosho, siya kuqhubeka ukuqhubela phambili umoya "womgangatho ophezulu, ukusebenza kakuhle, ukusungula izinto ezintsha, ingqibelelo", kwaye siqhubeke nomgaqo wokusebenza "wekhredithi ukuqalisa, umthengi ekuqaleni, umgangatho ophezulu obalaseleyo".Siza kwenza ixesha elide elimangalisayo kwimveliso yeenwele kunye namaqabane ethu.
Izinto zethu zichongwa kwaye zithenjwa ngabathengi kwaye zinokuzalisekisa ngokuqhubekayo ukuguqula iimfuno zezoqoqosho nezentlaloI-China Taq DNA Polymerase kunye ne-Qpcr, I-Item iphumelele ngesatifikethi sesizwe esiqinisekisiweyo kwaye yamkelwe kakuhle kwishishini lethu eliphambili.Iqela lethu lobunjineli bezakhono liya kuhlala likulungele ukukusebenzela ukuze uqhagamshelane kunye nempendulo.Sikwazile ukukuzisa ngeesampulu ezingahlawulelwayo ukuhlangabezana neenkcukacha zakho.Iinzame ezifanelekileyo ziya kuveliswa ukukunika eyona nkonzo iluncedo kunye nezisombululo.Ngaba unomdla kwinkampani yethu kunye nezisombululo, qiniseka ukuba unxibelelana nathi ngokusithumelela ii-imeyile okanye usitsalele umnxeba ngoko nangoko.Ukwazi izisombululo zethu kunye neshishini.ngaphezulu, uya kukwazi ukuza kumzi-mveliso wethu ukuze uyibone.Siza kuhlala samkela iindwendwe ezivela kwihlabathi liphela kwifemu yethu.o Yakha ishishini loshishino.uxolo kunye nathi.Nceda uzive ukhululekile ngokupheleleyo ukuthetha nathi ngombutho.kwaye sikholelwa ukuba siya kwabelana ngamava asebenzayo orhwebo nabo bonke abarhwebi bethu.

Iinkcazelo Kit

I-2 × I-PCR yangempela elula^TMUmxube-SYBR obonelelwa yi-Real Time PCR Easy^TM-SYBR Green I kit yinkqubo entsha ye-premix esebenzisa i-SYBR Green I yeReality Time PCR reactions yokukhulisa, enokuphucula kakhulu imveliso ethile kunye nobuntununtunu bokusabela.I-ROX inikezelwa njengedayi yolawulo lwangaphakathi.Ukuqina kwe-fluorescence yale khithi ngama-3-5 amaxesha eemveliso ezifanayo, ezinokuthi zibe novakalelo kwaye zibonise ngokucacileyo ukuxinwa kwe-template ye-DNA ekujoliswe kuyo.

I-2 × I-PCR yangempela elula^TMI-Mix-SYBR iqulethe i-Foregene eshushu-isiqalo esisodwa se-Taq DNA Polymerase.Xa kuthelekiswa nee-enzymes ze-Taq eziqhelekileyo, ineengenelo zokusebenza kwe-amplification ephezulu, isakhono esithile esinamandla sokukhulisa kunye nesantya esisezantsi sokungahambelani.Inokunciphisa ukukhuliswa okungangqaliyo kunye nokuphucula ukuchaneka kwe-PCR.

Iinkcukacha

Iimpawu&izinto eziluncedo

■ Ilula—I-2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza

■ I-Specific-optimized buffer kunye ne-hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.

■ Uvakalelo oluphezulu-lukwazi ukubona iikopi ezisezantsi zetemplate

■ Ukuguquguquka okuhle—okuhambelana nezixhobo ezininzi zePCR zexesha lokwenyani

Isicelo seKit

Uhlalutyo lwe-qPCR

Ukuhamba komsebenzi

Umzobo

Ukugcinwa kunye nobomi beshelufu

Le khithi kufuneka igcinwe kude nokukhanya kwaye kufuneka igcinwe ku -20 ℃.Ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ ixesha elifutshane (iintsuku ezili-10).

Izinto zethu zichongwa ngokuqhelekileyo kwaye zithenjwa ngabathengi kwaye zinokuzalisekisa ngokuqhubekayo ukutshintshela iimfuno zezoqoqosho kunye nezentlalo zeFactory yokwenza iLula kunye neXesha eliKhawulezayo le-PCR Kit kunye ne-High Rox + Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Ukususela ekubeni indawo yokuvelisa yasekwa, ngoku sizibophelele kwinkqubela phambili yeemveliso ezintsha.Ngelixa sisebenzisa isantya sentlalo kunye nezoqoqosho, siya kuqhubeka ukuqhubela phambili umoya "womgangatho ophezulu, ukusebenza kakuhle, ukusungula izinto ezintsha, ingqibelelo", kwaye siqhubeke nomgaqo wokusebenza "wekhredithi ukuqalisa, umthengi ekuqaleni, umgangatho ophezulu obalaseleyo".Siza kwenza ixesha elide elimangalisayo kwimveliso yeenwele kunye namaqabane ethu.
Ukwenziwa kwefektriI-China Taq DNA Polymerase kunye ne-Qpcr, I-Item iphumelele ngesatifikethi sesizwe esiqinisekisiweyo kwaye yamkelwe kakuhle kwishishini lethu eliphambili.Iqela lethu lobunjineli bezakhono liya kuhlala likulungele ukukusebenzela ukuze uqhagamshelane kunye nempendulo.Sikwazile ukukuzisa ngeesampulu ezingahlawulelwayo ukuhlangabezana neenkcukacha zakho.Iinzame ezifanelekileyo ziya kuveliswa ukukunika eyona nkonzo iluncedo kunye nezisombululo.Ngaba unomdla kwinkampani yethu kunye nezisombululo, qiniseka ukuba unxibelelana nathi ngokusithumelela ii-imeyile okanye usitsalele umnxeba ngoko nangoko.Ukwazi izisombululo zethu kunye neshishini.ngaphezulu, uya kukwazi ukuza kumzi-mveliso wethu ukuze uyibone.Siza kuhlala samkela iindwendwe ezivela kwihlabathi liphela kwifemu yethu.o Yakha ishishini loshishino.uxolo kunye nathi.Nceda uzive ukhululekile ngokupheleleyo ukuthetha nathi ngombutho.kwaye sikholelwa ukuba siya kwabelana ngamava asebenzayo orhwebo nabo bonke abarhwebi bethu.