Siyakwazi ukwanelisa ngokubanzi abathengi bethu abahlonitshwayo ngomgangatho wethu olungileyo kakhulu, ixabiso eliphezulu kunye nenkxaso entle kakhulu ngenxa yokuba siye sasebenza ngakumbi kwaye sisebenza nzima kwaye sikwenza ngendlela engabizi kakhulu kwiFactory ye-China yePlastiki yeMagnetic Bead ye-Universal Nucleic Acid Extraction Rapid Kit.Sijonge phambili kwintsebenziswano nawe kwixesha elizayo.
Siyakwazi ukwanelisa ngokubanzi abathengi bethu abahlonitshwayo ngomgangatho wethu olungileyo kakhulu, ixabiso eliphezulu kunye nenkxaso entle kakhulu ngenxa yokuba besinobuchwephesha obungakumbi kunye nokusebenza nzima okongezelelekileyo kwaye sikwenza ngendlela engabizi kakhulu.China Universal Nucleic Acid Extraction, IPlastiki yeNucleic Acid Extraction, Inkampani yethu ithobela imbono yolawulo "gcina izinto ezintsha, landela ukugqwesa".Ngesiseko sokuqinisekisa iingenelo zezisombululo ezikhoyo, siqhubeka sisomeleza kwaye sandisa uphuhliso lwemveliso.Inkampani yethu inyanzelisa ukusungula izinto ezintsha ukukhuthaza uphuhliso oluzinzileyo lweshishini, kwaye isenze sibe ngababoneleli abakumgangatho ophezulu basekhaya.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 ingagcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho).

Siyakwazi ukwanelisa ngokubanzi abathengi bethu abahlonitshwayo ngomgangatho wethu olungileyo kakhulu, ixabiso eliphezulu kunye nenkxaso entle kakhulu ngenxa yokuba siye sasebenza ngakumbi kwaye sisebenza nzima kwaye sikwenza ngendlela engabizi kakhulu kwiFactory ye-China yePlastiki yeMagnetic Bead ye-Universal Nucleic Acid Extraction Rapid Kit.Sijonge phambili kwintsebenziswano nawe kwixesha elizayo.
Umzi-mvelisoChina Universal Nucleic Acid Extraction, IPlastiki yeNucleic Acid Extraction, Inkampani yethu ithobela imbono yolawulo "gcina izinto ezintsha, landela ukugqwesa".Ngesiseko sokuqinisekisa iingenelo zezisombululo ezikhoyo, siqhubeka sisomeleza kwaye sandisa uphuhliso lwemveliso.Inkampani yethu inyanzelisa ukusungula izinto ezintsha ukukhuthaza uphuhliso oluzinzileyo lweshishini, kwaye isenze sibe ngababoneleli abakumgangatho ophezulu basekhaya.