Enekhredithi yeshishini elincinci elivakalayo, inkonzo enkulu emva kokuthengisa kunye nezixhobo zangoku zemveliso, sizuze ukuma okugqwesileyo phakathi kwabathengi bethu emhlabeni wonke ngokuSebenza okuPhezulu kweChina eSebenza ngokuLungileyo iTissue Genomic DNA Nucleic Acid Extraction Reagents, Kwimeko apho ujonge umgangatho ophezulu, ukuhanjiswa ngokukhawuleza, okona kulungileyo emva koncedo kunye nexabiso elilungileyo lokuya kumthengisi osebenza ixesha elide ukuya e-China, siyakukhetha ixesha elide.
Enetyala elivakalayo leshishini elincinci, inkonzo entle emva kokuthengisa kunye nezixhobo zale mihla zemveliso, sizuze ukuma okubalaseleyo phakathi kwabathengi bethu kwihlabathi liphela.I-Automatic Nucleic Acid Purification Extractor, China Tissue Genomic DNA Kit, Impahla yethu iphumelele igama elihle kakhulu kwilizwe ngalinye elinxulumeneyo.Ngenxa yokusekwa kwefemu yethu.sinyanzelise inkqubo yethu yokuvelisa izinto ezintsha kunye neyona ndlela yakutshanje yokulawula, sitsala ubuninzi beetalente kweli shishini.Sithatha isisombululo esisemgangathweni njengowona mlinganiswa ubalulekileyo wethu.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-5mg yenyama yempuku okanye i-2-5mm yomsila wempuku inokuvavanywa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza i-PCR ibenento ethile ephezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.

■ Inkqubo yePCR echasene nongcoliseko 2×M1-PCR Easy^TMUkuxuba (UNG), okuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

Iiparamitha zeKit

Ubungakanani besicelo: umsila wempuku, indlebe yempuku, isihlunu kunye nezinye izihlunu.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer AL inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

I-Reagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa kwi-4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-Reagent 2 × M1 PCR Easy TM Mix (UNG), ukuba isetyenziswa rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (ukusetyenziswa ngaphakathi kweentsuku ze-10) .Leyo inekhredithi yeshishini elincinci elivakalayo, inkonzo enkulu emva kokuthengisa kunye nezixhobo zemveliso zanamhlanje, sifumene ukuma okubalaseleyo phakathi kwabathengi bethu emhlabeni jikelele ngenxa ye-High Performance China i-Acvenient Exvenient Tissue i-Nuclear DNA ekhangele i-Nuclear DNA. umgangatho ophezulu, ukuhanjiswa ngokukhawuleza, okona kulungileyo emva koncedo kunye nomboneleli wexabiso elihle e-China uqhagamshelwano lwexesha elide lombutho, siya kuba lolona khetho lwakho lusebenzayo.
Ukwenza okuphezuluChina Tissue Genomic DNA Kit, I-Automatic Nucleic Acid Purification Extractor, Impahla yethu iphumelele igama elihle kakhulu kwilizwe ngalinye elinxulumeneyo.Ngenxa yokusekwa kwefemu yethu.sinyanzelise inkqubo yethu yokuvelisa izinto ezintsha kunye neyona ndlela yakutshanje yokulawula, sitsala ubuninzi beetalente kweli shishini.Sithatha isisombululo esisemgangathweni njengowona mlinganiswa ubalulekileyo wethu.