Sihlala sisenza ukuba singabasebenzi ababambekayo siqinisekisa ukuba siya kukunika elona xabiso libalaseleyo kunye nelona xabiso lilungileyo lokuthengisa kwintengiso eshushu ye-China PCR Nucleic Acid Test Kit eneFluorescent Rt PCR Test Method yeLab, wamkelekile amaqabane asuka mbombo zone zehlabathi ukuba uye kuyo, ngesandla kunye nothethathethwano.
Sidla ngokwenza ukuba singabasebenzi ababambekayo siqinisekisa ukuba siya kukunika elona ncedo lubalaseleyo kunye nelona xabiso lilungileyo lokuthengisaChina Diagnostic Test Kit, Uvavanyo olukhawulezileyo lokuxilonga, Ngenjongo "yokukhuphisana nomgangatho olungileyo kwaye uphuhlise ngokuyila" kunye nomgaqo wenkonzo "yokuthatha imfuno yabathengi njengoqhelaniso", siya kubonelela ngezisombululo ezifanelekileyo kunye nenkonzo elungileyo kubathengi basekhaya nabamazwe ngamazwe.

Iinkcukacha

50 Preps, 200 Preps

I-Viral RNA I-Nucleic Acid Ukucoca I-Isolation Kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA yentsholongwane kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid, kunye ne-cell culture supernatant.Ikiti yongeza ngokukodwa i-Linear Acrylamide, enokuthi ibambe ngokulula ixabiso elincinci le-RNA kwiisampuli.IKholam ye-RNA Kuphela inokubopha i-RNA ngokufanelekileyo.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Ikhithi yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer viRW1 kunye ne-Buffer viRW2 inokuqinisekisa ukuba i-viral nucleic acid efunyenweyo ayinaprotheyini, i-nuclease okanye enye into engcolileyo, enokusetyenziswa ngokuthe ngqo kwimifuniselo yebhayoloji yemolekyuli esezantsi.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Isivuno esiphezulu se-nucleic acid: I-DNA / RNA-iKholamu kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-DNA kunye ne-RNA.
■ Umthamo omkhulu wokusetyenzwa kwesampulu: ukuya kuthi ga kwi-200μl iisampulu zisenokwenziwa ngexesha ngalinye.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-20.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukukhutshwa kunye nokucocwa kwe-viral nucleic acid kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid kunye ne-cell culture supernatant.

Ukuhamba komsebenzi

Umzobo

Ugcino kunye nobomi beShelf

■ Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
■ Isisombululo seLinear Acrylamide sinokugcinwa kwiqondo lobushushu legumbi kangangeentsuku ezisi-7;emva kokufumana ikiti, nceda uyikhuphe kwaye uyigcine kwi-20°C.
■ Emva kokongeza i-Linear Acrylamide kwi-Buffer DRL, inokugcinwa kwi-2-8 ° C ukuya kwi-48h.Nceda usebenzise isisombululo esele senziwe.

Sihlala sisenza ukuba singabasebenzi ababambekayo siqinisekisa ukuba siya kukunika elona xabiso libalaseleyo kunye nelona xabiso lilungileyo lokuthengisa kwintengiso eshushu ye-China PCR Nucleic Acid Test Kit eneFluorescent Rt PCR Test Method yeLab, wamkelekile amaqabane asuka mbombo zone zehlabathi ukuba uye kuyo, ngesandla kunye nothethathethwano.
Intengiso eshushu yeChina Diagnostic Test Kit, Uvavanyo olukhawulezileyo lokuxilonga, Ngenjongo "yokukhuphisana nomgangatho olungileyo kwaye uphuhlise ngokuyila" kunye nomgaqo wenkonzo "yokuthatha imfuno yabathengi njengoqhelaniso", siya kubonelela ngezisombululo ezifanelekileyo kunye nenkonzo elungileyo kubathengi basekhaya nabamazwe ngamazwe.