Sinabasebenzi bethu bokuthengisa imveliso, abasebenzi besitayile, iqela lobugcisa, abasebenzi be-QC kunye nabasebenzi bephakheji.Ngoku sineenkqubo ezingqongqo zolawulo lomgangatho ophezulu kwindlela nganye.Kwakhona, bonke abasebenzi bethu banamava kumxholo woshicilelo we-OEM/ODM ManufacturerChina Nucleic Acid Extraction Reagent(I-Serum / i-Plasma kunye nesisombululo sePuncture) , Iikhithi zoVavanyo lwe-Rna Extraction, Injongo yethu yokuvala "Ukuzama eyona nto inenzuzo kakhulu, Ngokubanzi iyona nto ingcono kakhulu".Qiniseka ukuba uziva simahla ukuze udibane nathi ukuba unazo naziphi na izinto ezifunekayo.
Sinabasebenzi bethu bokuthengisa imveliso, abasebenzi besitayile, iqela lobugcisa, abasebenzi be-QC kunye nabasebenzi bephakheji.Ngoku sineenkqubo ezingqongqo zolawulo lomgangatho ophezulu kwindlela nganye.Kwakhona, bonke abasebenzi bethu banamava kumbandela wokushicilelaChina Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Umgaqo wethu "yimfezeko kuqala, umgangatho ophezulu".Ngoku sinethemba lokubonelela ngenkonzo ebalaseleyo kunye nezinto ezifanelekileyo.Sithemba ngokunyanisekileyo ukuba singaseka intsebenziswano yokuphumelela ishishini nawe kwixesha elizayo!

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ inyanga ye-1 emva kokufaka i-β- mercaptoethanol (ukhetho) .Sinabasebenzi bethu bokuthengisa imveliso, abasebenzi besitayela, iqela lobugcisa, abasebenzi be-QC kunye nabasebenzi bephakheji.Ngoku sineenkqubo ezingqongqo zolawulo lomgangatho ophezulu kwindlela nganye.Kwakhona, bonke abasebenzi bethu banamava kumxholo woshicilelo we-OEM/ODM ManufacturerChina Nucleic Acid Extraction Reagent(I-Serum / i-Plasma kunye nesisombululo sePuncture) , Iikhithi zoVavanyo lwe-Rna Extraction, Injongo yethu yokuvala "Ukuzama eyona nto inenzuzo kakhulu, Ngokubanzi iyona nto ingcono kakhulu".Qiniseka ukuba uziva simahla ukuze udibane nathi ukuba unazo naziphi na izinto ezifunekayo.
Umvelisi we-OEM/ODM waseTshayina iNucleic Acid yokutsalwa kweReagent,Indlela ye-Magnetic Bead, Umgaqo wethu "yimfezeko kuqala, umgangatho ophezulu".Ngoku sinethemba lokubonelela ngenkonzo ebalaseleyo kunye nezinto ezifanelekileyo.Sithemba ngokunyanisekileyo ukuba singaseka intsebenziswano yokuphumelela ishishini nawe kwixesha elizayo!