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Enye yezona zishushu kakhulu kwi-China Vtm Factory ethengisa ngokuthe ngqo iSampuli yeSampule yeSampuli yokuqokelelwa kweSampuli eLahlwayo

Enye yezona zishushu kakhulu kwi-China Vtm Factory ethengisa ngokuthe ngqo iSampuli yeSampule yeSampuli yokuqokelelwa kweSampuli eLahliweyo yeThubhu eFakelwe umfanekiso
  • Enye yezona zishushu kakhulu kwi-China Vtm Factory ethengisa ngokuthe ngqo iSampuli yeSampule yeSampuli yokuqokelelwa kweSampuli eLahlwayo
  • Enye yezona zishushu kakhulu kwi-China Vtm Factory ethengisa ngokuthe ngqo iSampuli yeSampule yeSampuli yokuqokelelwa kweSampuli eLahlwayo
  • Enye yezona zishushu kakhulu kwi-China Vtm Factory ethengisa ngokuthe ngqo iSampuli yeSampule yeSampuli yokuqokelelwa kweSampuli eLahlwayo

Inkcazelo yeKit:

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo ukukhulula ngokukhawuleza i-DNA ye-genomic kwiisampuli zezicubu zezilwanyana ukwenzela ukusabela kwe-PCR, ngoko ke ifaneleke ngokukodwa kuvavanyo olukhulu lwemfuzo.

Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwe ngaphakathi kwe-10-30 imizuzu kwi-65.°C. Azikho ezinye iinkqubo ezifana neprotheyini kunye nokususwa kwe-RNA ezifunekayo, kunye ne-DNA trace ekhutshiweyo ingasetyenziswa njenge template ye-PCR reaction.

2×PCR EasyTMUmxube (UNG) usebenzisa i-dUTP endaweni ye-dTTP kwisiseko se-2×PCR EasyTMHlanganisa, kwaye ungeze i-enzyme ye-UNG (i-Uracil-N-glycosylase) enokunciphisa i-template equkethe i-dUTP ngexesha elifanayo.Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthotywa kwemveliso ye-PCR equkethe i-uracil.I-enzyme ye-UNG ayiyi kuba nayiphi na impembelelo kwi-template engenawo i-uracil, ngaloo ndlela iqinisekisa ukuchaneka kunye nokuchaneka kwe-amplification kunye nokuthintela ukuba kungenzeka ukuba ukungcola kweemveliso ze-PCR ngexesha lovavanyo olukhulu lofuzo.

I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Eyona nto esijolise kuyo iya kuba kukubonelela abathengi bethu ngobudlelwane obunobuzaza kunye nonoxanduva lobudlelwane bamashishini amancinci, ukubonelela ngengqwalasela yobuqu kubo bonke kweyeyona iHottest ye-China Vtm Factory Direct Selling Virus Sample Collection Disposable Sampling Tube, Okwangoku, igama lombutho lineentlobo ezininzi zeemveliso ezingaphezu kwama-4000 kwaye lifumene iwonga elihle kakhulu kunye nezabelo ezinkulu kwimarike yangoku yasekhaya nakwamanye amazwe.
Eyona njongo yethu iphambili iya kuba kukubonelela abathengi bethu ngobudlelwane obunzulu kunye nonoxanduva lwamashishini amancinci, sinikezela ingqalelo yobuqu kubo bonke.Ityhubhu yeSampulu eLahliweyo yaseTshayina, Ityhubhu yoQokelelo lweSampulu, Singalamkela kakhulu ithuba lokwenza ishishini nawe kwaye siyonwabele ukuncamathelisa iinkcukacha ezithe vetshe zentengiso yethu.Umgangatho ogqwesileyo, amaxabiso akhuphisanayo, unikezelo ngexesha kunye nenkonzo ethembekileyo inokuqinisekiswa.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I

Isithinteli AL

I-Foregene Protease

I-6 × i-DNA yokulayisha i-Buffer

Icandelo II

2×PCR EasyTMUmxube(UNG)

Iimpawu&izinto eziluncedo

■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi

∎ Imfuno yesampulu incinci, kancinane njenge-5mg inyama yesilwanyana inokuvavanywa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

■ Inkqubo yePCR yokuchasana nongcoliseko 2×PCR EasyTMUkuxuba (UNG) kuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kwaye iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

Iiparamitha zeKit

Isicelo: iintlobo ezahlukeneyo zezicubu zezilwanyana.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

wk-flw-isilwanyana kwi-PCR ngqo

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer AL inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

I-Reagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa kwi-4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃ .

I-Reagent 2 × PCR EasyTMMix(UNG), ukuba isetyenziswa rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (ukusetyenziswa ngaphakathi kweentsuku ze-10) .Injongo yethu ephambili iya kuba kukubonelela abathengi bethu ngobudlelwane boshishino oluncinci kunye noxanduva, ukubonelela ingqalelo yobuqu kubo bonke ngenxa ye-One of Hottest ye-China Vtm Factory Direct Selling Virus Sample Collection Collections I-Sampling Disposable Disposable 0 i-Sampling Tube kunye ne-0 ilungile kakhulu i-Sampling Tube kunye negama le-0 Corporation. ubume kunye nezabelo ezinkulu kwimarike yangoku yangaphakathi nangaphandle.
Enye yeHottest yeItyhubhu yeSampulu eLahliweyo yaseTshayina, Ityhubhu yoQokelelo lweSampulu, Singalamkela kakhulu ithuba lokwenza ishishini nawe kwaye siyonwabele ukuncamathelisa iinkcukacha ezithe vetshe zentengiso yethu.Umgangatho ogqwesileyo, amaxabiso akhuphisanayo, unikezelo ngexesha kunye nenkonzo ethembekileyo inokuqinisekiswa.

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