Ukudala ixabiso elingakumbi kubathengi yifilosofi yethu yoshishino;Ukukhula komthengi kukuleqa kwethu ukusebenza kwe-plasmid dna kits yegumbi lobushushu bokusebenza spin ngokukhawuleza kwaye kulula, Sikholelwa ukuba iqela elinomdla, eliguqulayo kunye neqela eliqeqeshwe kakuhle kufuneka likwazi ukuseka ubudlelwane beshishini obulungileyo kunye noluncedo ngokufanayo kunye nawe kungekudala.Khumbula ukuziva kungekho ndleko yokunxibelelana nathi ukuze ufumane ezinye iinyani.
Ukudala ixabiso elingakumbi kubathengi yifilosofi yethu yoshishino;ukukhula kwabathengi kukuleqa kwethu ukusebenzaChina DNA Extract, Dna Isolation, Ukusukela rhoqo, sibambelela "ekuvulekeni kunye nokulunga, ukwabelana ukuze sifumane, ukufuna ukugqwesa, kunye nokudalwa kwexabiso"ixabiso, sithobela "ingqibelelo kunye nokusebenza kakuhle, ukurhweba, indlela engcono kakhulu, i-valve engcono" ifilosofi yoshishino.Sikunye nelizwe lethu jikelele sinamasebe kunye namahlakani okuphuhlisa iindawo zamashishini amatsha, amaxabiso afanayo aphezulu.Samkela ngokunyanisekileyo kwaye kunye sabelana ngezixhobo zehlabathi, sivula ikhondo elitsha kunye nesahluko.

Iinkcazelo Kit

I-FOREGENE'S nucleic acid extraction kunye neemveliso zechungechunge zokucoca zinokukhutshwa kwaye zihlanjululwe kwizinga eliphezulu le-nucleic acids ezivela kwimithombo eyahlukeneyo kunye neesampuli zamacandelo anzima.Iimveliso ze-DNA extraction series zisekelwe kwi-DNA-Kuphela i-silica membrane ye-silica iikholamu zokuhlanjululwa kunye ne-reagents ye-formula eyodwa enokuthi ihlambulule i-DNA ephezulu kwiisampuli ezahlukeneyo.

I-General Plasmid Mini Kit isebenzisa iteknoloji yekholomu yokucoca i-DNA-Kuphela kunye nefomula ye-SDS ye-lysis esebenzayo yokufumana i-plasmid DNA ephezulu kwiibhaktheriya ngaphakathi kwemizuzu engama-20.I-DNA-Kuphela i-silica gel membrane esetyenziswe kwikholamu ye-spin yinto entsha ekhethekileyo ye-FOREGENE, enokuthi ibhengeze i-DNA ngokufanelekileyo kwaye ngokuthe ngqo, kwaye isuse i-RNA, iiprotheni ezingcolileyo, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba elikhulu.Umthamo omkhulu wokubopha i-plasmid DNA yekholomu yokucoca kuphela yi-60μg DNA.Ubungakanani be-plasmid DNA ekhutshwe ngokwenene ihambelana nomthamo wesisombululo sebhaktheriya, inani leekopi ze-plasmid (ikopi ephezulu, ikopi ephantsi), ubunzima, iimeko zenkcubeko kunye nezinye izinto.

Iinkcukacha

Iimpawu&izinto eziluncedo

- Akukho ngcoliseko ye-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-plasmid DNA ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuphepha ukungcoliseka kwebhubhoratri nge-RNase yangaphandle.

- Isantya esikhawulezayo: I-Plasmid DNA extraction ingagqitywa kwimizuzu engama-20.

- Ikhuselekile: Akukho kutsalwa kwe-organic reagent efunekayo.

- Umgangatho ophezulu: I-plasmid efunyenwe ngokutsalwa ayinayo i-RNA, ayikho i-RNase kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

Isicelo seKit

I-Plasmid DNA extraction kunye nokuhlanjululwa kweebhaktheriya ze-Gram-negative.

Ukuhamba komsebenzi

Umzobo

Ukugcinwa kunye nobomi beshelufu

Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.

Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yifudumeze kwibhafu yamanzi engama-37 ℃ imizuzu eyi-10 ukunyibilikisa imvula, kwaye uyixube phambi kokusetyenziswa.

Ukudala ixabiso elingakumbi kubathengi yifilosofi yethu yoshishino;ukukhula kwabathengi kukuleqa kwethu ukusebenza kubathengisi beWholesale baseTshayina 96automated Nucleic Acid Extraction System yePCR Sampling Preparation, Sikholelwa ukuba iqela elinomdla, eliguqulayo neliqeqeshwe kakuhle lifanele likwazi ukuseta ubudlelwane beshishini obulunge kakhulu nobuluncedo kunye nawe kungekudala.Khumbula ukuziva kungekho ndleko yokunxibelelana nathi ukuze ufumane ezinye iinyani.
Abathengisi beWholesale yeChina DNA Extract, DNA Isolation, Ukususela rhoqo, sibambelele "evulekileyo kwaye enobulungisa, ukwabelana ukufumana, ukufuna ukugqwesa, kunye nokudala ixabiso"amaxabiso, ukubambelela "ingqibelelo kunye esebenzayo, urhwebo-oriented, eyona ndlela , best valve" ifilosofi ishishini.Sikunye nelizwe lethu jikelele sinamasebe kunye namahlakani okuphuhlisa iindawo zamashishini amatsha, amaxabiso afanayo aphezulu.Samkela ngokunyanisekileyo kwaye kunye sabelana ngezixhobo zehlabathi, sivula ikhondo elitsha kunye nesahluko.