Iikowuteshini ezikhawulezayo nezigqwesileyo, abacebisi abanolwazi ukukunceda ukhethe imveliso echanekileyo ehambelana nazo zonke iimfuno zakho, ixesha elifutshane lokuvelisa, ulawulo oluphezulu olunoxanduva kunye neenkonzo ezizodwa zokuhlawula kunye nemicimbi yokuthumela ngexabiso elicatshulwe eTshayina.Cofa-It Edu-647IKit yokuFumana iCell Proliferation, Ishishini lokuqala, siyavana.Ishishini elongezelelweyo, intembeko iyafika apho.Ifemu yethu ihlala ikwinkonzo yakho nangaliphi na ixesha.
Iikowuti ezikhawulezayo nezigqwesileyo, abacebisi abanolwazi ukukunceda ukhethe imveliso echanekileyo ehambelana nazo zonke iimfuno zakho, ixesha elifutshane lokuvelisa, ulawulo oluphezulu olunoxanduva kunye neenkonzo ezizodwa zokuhlawula kunye nemicimbi yokuthumela ngenqanawa.Ikhithi yokuFumana iSeli yaseTshayina, Cofa-It Edu-647, Iimveliso zethu zibonwa ngokubanzi kwaye zithenjwa ngabasebenzisi kwaye zinokuhlangabezana ngokuqhubekayo nokutshintsha kweemfuno zoqoqosho nezentlalo.Samkela abathengi abatsha nabadala abavela kuzo zonke iinkalo zobomi ukuba baqhagamshelane nathi kubudlelwane beshishini elizayo kunye nempumelelo efanayo!

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa nge-4 ℃ i-1 ℃ inyanga enye emva kongeza i--Merlucaptoethanol (Inketho yemveliso enoxanduva lokufumana i-China eCelayo, i-Instorical Spenses, siqondana.Ishishini elongezelelweyo, intembeko iyafika apho.Ifemu yethu ihlala ikwinkonzo yakho nangaliphi na ixesha.
Ixabiso elicatshulweyo leIkhithi yokuFumana iSeli yaseTshayina, Cofa-It Edu-647, Iimveliso zethu zibonwa ngokubanzi kwaye zithenjwa ngabasebenzisi kwaye zinokuhlangabezana ngokuqhubekayo nokutshintsha kweemfuno zoqoqosho nezentlalo.Samkela abathengi abatsha nabadala abavela kuzo zonke iinkalo zobomi ukuba baqhagamshelane nathi kubudlelwane beshishini elizayo kunye nempumelelo efanayo!