Siqhubeka nomoya wethu weshishini "woMgangatho, uMsebenzi, uTshintsho kunye neMfezeko".Sijonge ukwenza ixabiso elithe kratya kubathengi bethu ngezixhobo zethu ezilayishiweyo, oomatshini banamhlanje, abasebenzi abanamava kunye neenkonzo ezinkulu zeengcaphephe zexabiso eliKhethekileyo leChina leXesha elililo lobungakanani bePCR Thermal Cycler ene-10.4 Intshi egcweleyo yoMbala weSkrini sokuchukumisa, ugxininiso olukhethekileyo ekupakishweni kwezinto ezithengiswayo ukuphepha nawuphi na umonakalo ngexesha lothutho, Iingcebiso ezithe ngqo kubathengi bethu.
Siqhubeka nomoya wethu weshishini "woMgangatho, uMsebenzi, uTshintsho kunye neMfezeko".Sijonge ukwenza ixabiso elithe chatha kubathengi bethu ngezixhobo zethu ezilayishiweyo, oomatshini banamhlanje, abasebenzi abanamava kunye neenkonzo ezinkulu zeengcaliChina Portabl PCR Thermal Cycler, I-Thermal Cycler kwi-PCR Machine, Ngomgangatho omhle, ixabiso elifanelekileyo kunye nenkonzo enyanisekileyo, sinandipha igama elihle.Izinto ezithengiswayo zithunyelwa eMzantsi Melika, eOstreliya, eMzantsi-mpuma Asia njalo njalo.Bamkele ngokufudumeleyo abathengi ekhaya nakwamanye amazwe ukusebenzisana nathi ngekamva eliqaqambileyo.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-1mg ye-caudal fin yentlanzi okanye amaqanda entlanzi ali-10 anokuhlolwa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ye-Anti-pollution 2 × PCR Easy TM Mix (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Iintlanzi zeZebra kunye nezinye iintlanzi zamanzi ahlambulukileyo.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

v IReagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

vReagent 6×DNA Loading Buffer ingagcinwa kwi 4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-vReagent 2 × PCR Easy TM Mix (UNG), ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (ukusetyenziswa ngaphakathi kweentsuku ze-10) .Siqhubeka nomoya wethu weshishini "Ubunjani, ukuSebenza, ukuQala ngokutsha kunye noBunyanisekileyo".Sijonge ukwenza ixabiso elithe kratya kubathengi bethu ngezixhobo zethu ezilayishiweyo, oomatshini banamhlanje, abasebenzi abanamava kunye neenkonzo ezinkulu zeengcaphephe zexabiso eliKhethekileyo leChina leXesha elililo lobungakanani bePCR Thermal Cycler ene-10.4 Intshi egcweleyo yoMbala weSkrini sokuchukumisa, ugxininiso olukhethekileyo ekupakishweni kwezinto ezithengiswayo ukuphepha nawuphi na umonakalo ngexesha lothutho, Iingcebiso ezithe ngqo kubathengi bethu.
Ixabiso elikhethekileyo leChina Portabl PCR Thermal Cycler, I-Thermal Cycler kwi-PCR Machine, Ngomgangatho omhle, ixabiso elifanelekileyo kunye nenkonzo enyanisekileyo, sinandipha igama elihle.Izinto ezithengiswayo zithunyelwa eMzantsi Melika, eOstreliya, eMzantsi-mpuma Asia njalo njalo.Bamkele ngokufudumeleyo abathengi ekhaya nakwamanye amazwe ukusebenzisana nathi ngekamva eliqaqambileyo.