Sihlala sicinga kwaye siziqhelanisa ngokuhambelana nokutshintsha kweemeko, kwaye sikhule.Sinenjongo yokuphumelela kwengqondo kunye nomzimba otyebileyo kunye nokuphila kuYilo oluKhethekileyo lweYunivesithi yaseTshayina yoSetyenziso lwePhepha lePCR elisezantsi lokuLinga i-Automated Nucleic Acid Extractor, Ubukho obuqhubekayo bezinto zodidi oluphezulu kudityaniswe nenkxaso yethu ekhethekileyo yaphambi nasemva kokuthengisa iqinisekisa ukhuphiswano olomeleleyo kwindawo yentengiso yehlabathi.
Sihlala sicinga kwaye siziqhelanisa ngokuhambelana nokutshintsha kweemeko, kwaye sikhule.Sinenjongo ekufezekiseni ingqondo nomzimba otyebileyo kunye nokuphilaI-Automatic Nucleic Acid Extractor, China I-Automatic Nucleic Acid Extractor, Inkampani yethu yakhe ubudlelwane beshishini obuzinzileyo kunye neenkampani ezininzi ezaziwayo zasekhaya kunye nabathengi baphesheya kolwandle.Ngenjongo yokubonelela ngeemveliso ezikumgangatho ophezulu kunye nezisombululo kubathengi kwiibhedi eziphantsi, sizibophelele ekuphuculeni amandla ayo kuphando, uphuhliso, ukuvelisa kunye nokulawula.Sinelunda ukufumana ulwamkelo kubathengi bethu.Ukuza kuthi ga ngoku siphumelele i-ISO9001 ngo-2005 kunye ne-ISO/TS16949 ngo-2008. Amashishini "omgangatho wokuphila, intembeko yophuhliso" ngenjongo, amkele ngokunyanisekileyo oosomashishini basekhaya nabangaphandle ukuba batyelele ukuze baxoxe ngentsebenziswano.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sihlala sicinga kwaye siziqhelanisa ngokuhambelana nokutshintsha kweemeko, kwaye sikhule.Sinenjongo yokuphumelela kwengqondo kunye nomzimba otyebileyo kunye nokuphila kuYilo oluKhethekileyo lweYunivesithi yaseTshayina yoSetyenziso lwePhepha lePCR elisezantsi lokuLinga i-Automated Nucleic Acid Extractor, Ubukho obuqhubekayo bezinto zodidi oluphezulu kudityaniswe nenkxaso yethu ekhethekileyo yaphambi nasemva kokuthengisa iqinisekisa ukhuphiswano olomeleleyo kwindawo yentengiso yehlabathi.
Uyilo olukhethekileyo lweChina I-Automatic Nucleic Acid Extractor, I-Automatic Nucleic Acid Extractor, Inkampani yethu yakhe ubudlelwane beshishini obuzinzileyo kunye neenkampani ezininzi ezaziwayo zasekhaya kunye nabathengi baphesheya kolwandle.Ngenjongo yokubonelela ngeemveliso ezikumgangatho ophezulu kunye nezisombululo kubathengi kwiibhedi eziphantsi, sizibophelele ekuphuculeni amandla ayo kuphando, uphuhliso, ukuvelisa kunye nokulawula.Sinelunda ukufumana ulwamkelo kubathengi bethu.Ukuza kuthi ga ngoku siphumelele i-ISO9001 ngo-2005 kunye ne-ISO/TS16949 ngo-2008. Amashishini "omgangatho wokuphila, intembeko yophuhliso" ngenjongo, amkele ngokunyanisekileyo oosomashishini basekhaya nabangaphandle ukuba batyelele ukuze baxoxe ngentsebenziswano.