Ithagethi yethu ihlala ikwanelisa abathengi bethu ngokunikezela ngenkxaso yegolide, ixabiso eliphezulu kunye nomgangatho ophezulu kuMvelisi oKhokelayo waseTshayina iRna yeViral & DNA Extraction Kit yeSpin Indlela yeKholamu, Sinethemba elinyanisekileyo lokubonelela wena kunye nenkampani yakho ngesiqalo esihle.Ukuba kukho nantoni na esiya kuyenza ukuze ihambelane neemfuno zakho, siya kongezwa kunokonwabela ukwenza njalo.Wamkelekile kwiziko lethu lemveliso ukuba umise.
Ujoliso lwethu luhlala lukwanelisa abathengi bethu ngokunikela ngenkxaso yegolide, ixabiso eliphezulu kunye nomgangatho ophezulu weChina Rna & DNA Extraction Kit, I-Nucleic Acid Extraction, Injongo yethu kukunceda abathengi baqonde iinjongo zabo.Besisenza iinzame ezinkulu zokufezekisa le meko yokuphumelela kwaye sikwamkela ngokunyanisekileyo ukuba usijoyine.Ngelizwi, xa usikhetha, ukhetha ubomi obugqibeleleyo.Wamkelekile ukutyelela umzi-mveliso wethu kwaye wamkele iodolo yakho!Ngemibuzo engaphezulu, akufanele ungathandabuzi ukuqhagamshelana nathi.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 ingagcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-β- mercaptoethanol (ukhetho) .Injongo yethu ihlala iyanelisa abathengi bethu ngokunikela ngenkxaso yegolide, ixabiso eliphezulu kunye nomgangatho ophezulu kuMvelisi oPhambili we-China Viral Rna & DNA Extraction Kit of Spin Column Method, Siqala ngokunyanisekileyo ukubonelela ngenkampani yakho kwaye sithemba ngokunyanisekileyo ukubonelela ngenkampani yakho.Ukuba kukho nantoni na esiya kuyenza ukuze ihambelane neemfuno zakho, siya kongezwa kunokonwabela ukwenza njalo.Wamkelekile kwiziko lethu lemveliso ukuba umise.
UMvelisi oKhokelayo weRna yaseTshayina & neKhithi yokutsalwa kweDNA,I-Nucleic Acid Extraction, Injongo yethu kukunceda abathengi baqonde iinjongo zabo.Besisenza iinzame ezinkulu zokufezekisa le meko yokuphumelela kwaye sikwamkela ngokunyanisekileyo ukuba usijoyine.Ngelizwi, xa usikhetha, ukhetha ubomi obugqibeleleyo.Wamkelekile ukutyelela umzi-mveliso wethu kwaye wamkele iodolo yakho!Ngemibuzo engaphezulu, akufanele ungathandabuzi ukuqhagamshelana nathi.