Sinale motto engqondweni, ngokuqinisekileyo siye saba ngomnye wabona bavelisa izinto ezintsha ngokwethekhinoloji, abangabizi kakhulu, kunye nabavelisi abakhuphisana ngamaxabiso kuBanga oluPhezulu.China Nucleic Acid Extractionokanye I-Purification Kit spin indlela yekholamu, Ngoku sele sisebenza iminyaka engaphezu kwe-10.Sizinikezele kwiimveliso ezikumgangatho ophezulu kunye nezisombululo kunye noncedo lwabathengi.Siyakumema ukuba ngokuqinisekileyo uyeke kwishishini lethu kukhenketho olulolwakho kunye nesikhokelo senkampani esiphucukileyo.
Sinale motto engqondweni, ngokuqinisekileyo siye saba ngomnye wabona benzi bobuchwepheshe bavelisa izinto ezintsha, abaneendleko eziphantsi, nabakhuphisana ngamaxabiso ezinto.China Nucleic Acid Extraction, spin ikholamu Indlela, Sakhe ubudlelwane obuqinileyo kunye nobude bentsebenziswano kunye nobuninzi beenkampani ngaphakathi kweli shishini phesheya.Inkonzo yangoko nangoko emva kokuthengisa ebonelelwa liqela lethu labacebisi bonwabile abathengi bethu.Ubunzulu Ulwazi kunye neeparameters ezivela kwintengiso mhlawumbi ziya kuthunyelwa kuwe nakuphi na ukuvuma ngokucokisekileyo.Iisampulu zasimahla zinokuziswa kwaye inkampani ijonge kwiqumrhu lethu.n IPortugal yothethathethwano yamkelekile rhoqo.Ndiyathemba ukufumana imibuzo kukuchwetheza kwaye wakhe intsebenziswano yexesha elide.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa ku-4 ℃ kangangenyanga enye emva kokongeza i-2-hydroxy-1-ethanethiol(ukhetho). Unale motto engqondweni, ngokuqinisekileyo siye saba ngomnye wabona benzi bobuchwepheshe buvelisa izinto ezintsha, bonga iindleko, nabakhuphisana ngamaxabiso kuBanga oluPhezulu.China Nucleic Acid Extractionokanye iKhiti yokuhlanjululwa (iNdlela yeMagnetic Bead), Ngoku sele sisebenza iminyaka engaphezu kwe-10.Sizinikezele kwiimveliso ezikumgangatho ophezulu kunye nezisombululo kunye noncedo lwabathengi.Siyakumema ukuba ngokuqinisekileyo uyeke kwishishini lethu kukhenketho olulolwakho kunye nesikhokelo senkampani esiphucukileyo.
IBanga eliPhezulu laseTshayina lokuCinywa kwe-Asidi yeNyukliya, iNdlela yeMaso yeMagnetic, sakhe ubudlelwane obuluqilima nobude bentsebenziswano kunye nomthamo omkhulu weenkampani kweli shishini phesheya kolwandle.Inkonzo yangoko nangoko emva kokuthengisa ebonelelwa liqela lethu labacebisi bonwabile abathengi bethu.Ubunzulu Ulwazi kunye neeparameters ezivela kwintengiso mhlawumbi ziya kuthunyelwa kuwe nakuphi na ukuvuma ngokucokisekileyo.Iisampulu zasimahla zinokuziswa kwaye inkampani ijonge kwiqumrhu lethu.n IPortugal yothethathethwano yamkelekile rhoqo.Ndiyathemba ukufumana imibuzo kukuchwetheza kwaye wakhe intsebenziswano yexesha elide.