Iimveliso zethu zithathwa ngokubanzi kwaye zithembekile ngabasebenzisi bokugqibela kwaye zinokuhlangabezana neemfuno eziguqukayo zemali nezentlalo kwiiFactory Outlets China Baybio Bbex32 High Efficiency Automated Nucleic Acid Extraction Platform Izixhobo zoNyango, abathengi bethu basasazwa ikakhulu kuMntla Melika, eAfrika naseMpuma Yurophu.siyakwazi ukufumana izisombululo ezikumgangatho ophezulu kunye nexabiso elihle elinobundlobongela.
Iimveliso zethu zijongwa ngokubanzi kwaye zithembeke ngabasebenzisi bokugqibela kwaye zinokuhlangabezana neemfuno eziguqukayo zemali nezentlaloI-Automatic Nucleic Acid Purification Extractor, China Nucleic Acid Extractor, Inkampani yethu inikezela ngoluhlu olupheleleyo ukusuka kwintengiso yangaphambili ukuya kwinkonzo yasemva kokuthengisa, ukusuka kuphuhliso lwemveliso ukuya kuphicotho lokusetyenziswa kwesondlo, ngokusekelwe kumandla obugcisa obuqinileyo, ukusebenza kwemveliso ephezulu, amaxabiso afanelekileyo kunye nenkonzo egqibeleleyo, siya kuqhubeka nokuphuhlisa, ukuhambisa izinto ezikumgangatho ophezulu kunye neenkonzo, kunye nokukhuthaza intsebenziswano ehlala ihleli kunye nabathengi bethu, uphuhliso olufanayo kunye nokudala ikamva elingcono.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Iimveliso zethu zithathwa ngokubanzi kwaye zithembekile ngabasebenzisi bokugqibela kwaye zinokuhlangabezana neemfuno eziguqukayo zemali nezentlalo kwiiFactory Outlets China Baybio Bbex32 High Efficiency Automated Nucleic Acid Extraction Platform Izixhobo zoNyango, abathengi bethu basasazwa ikakhulu kuMntla Melika, eAfrika naseMpuma Yurophu.siyakwazi ukufumana izisombululo ezikumgangatho ophezulu kunye nexabiso elihle elinobundlobongela.
Iivenkile zoMzi-mvelisoChina Nucleic Acid Extractor, I-Automatic Nucleic Acid Purification Extractor, Inkampani yethu inikezela ngoluhlu olupheleleyo ukusuka kwintengiso yangaphambili ukuya kwinkonzo yasemva kokuthengisa, ukusuka kuphuhliso lwemveliso ukuya kuphicotho lokusetyenziswa kwesondlo, ngokusekelwe kumandla obugcisa obuqinileyo, ukusebenza kwemveliso ephezulu, amaxabiso afanelekileyo kunye nenkonzo egqibeleleyo, siya kuqhubeka nokuphuhlisa, ukuhambisa izinto ezikumgangatho ophezulu kunye neenkonzo, kunye nokukhuthaza intsebenziswano ehlala ihleli kunye nabathengi bethu, uphuhliso olufanayo kunye nokudala ikamva elingcono.