Ukubambelela kumgaqo wakho othi "Umgangatho oMhle kakhulu, inkonzo eyanelisayo", sizama ukufumana iqabane leshishini elimangalisayo kuwe ngeXabiso eliNgcono eTshayina eLitsha eliKhawulezayo lokwenyani ixesha loVavanyo lwePCR loMatshini weXabiso elithengiswayo kunye neSatifikethi seCE, iimpahla eziyilwe ngenzuzo yophawu.Sizimasa ngokuzimisela ukuvelisa nokuziphatha ngemfezeko, kwaye sithandeka kubathengi basekhaya naphesheya koshishino lwe-xxx.
Ukubambelela kumgaqo wakho "womgangatho oMhle kakhulu, inkonzo eyanelisayo", sizama ukufumana iqabane leshishini elimangalisayo kuwe.Ukufunyanwa kwe-Acid ye-Nucleic ngokuzenzekelayo, China Rt PCR Izixhobo zokubona, Igama leNkampani, lihlala limalunga nomgangatho njengesiseko senkampani, ifuna uphuhliso ngeqondo eliphezulu lokuthembeka, ukuthobela umgangatho wolawulo lwe-ISO ngokungqongqo, ukudala inkampani ekwinqanaba eliphezulu ngomoya wokuthembeka nokuphawula inkqubela phambili nethemba.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Ukubambelela kumgaqo wakho othi "Umgangatho oMhle kakhulu, inkonzo eyanelisayo", sizama ukufumana iqabane leshishini elimangalisayo kuwe ngeXabiso eliNgcono eTshayina eLitsha eliKhawulezayo lokwenyani ixesha loVavanyo lwePCR loMatshini weXabiso elithengiswayo kunye neSatifikethi seCE, iimpahla eziyilwe ngenzuzo yophawu.Sizimasa ngokuzimisela ukuvelisa nokuziphatha ngemfezeko, kwaye sithandeka kubathengi basekhaya naphesheya koshishino lwe-xxx.
Ixabiso elingcono kakhuluChina Rt PCR Izixhobo zokubona, Ukufunyanwa kwe-Acid ye-Nucleic ngokuzenzekelayo, Igama leNkampani, lihlala limalunga nomgangatho njengesiseko senkampani, ifuna uphuhliso ngeqondo eliphezulu lokuthembeka, ukuthobela umgangatho wolawulo lwe-ISO ngokungqongqo, ukudala inkampani ekwinqanaba eliphezulu ngomoya wokuthembeka nokuphawula inkqubela phambili nethemba.