Sikholelwa ukuba intsebenziswano yokuvakalisa ixesha elide eneneni sisiphumo somgangatho ophezulu, iinkonzo ezixabisa ukongezwa, amava anempumelelo kunye noqhagamshelwano lobuqu lweZitishi zeVirus Magnetic Bead Indlela ye-Rna-DNA Nucleic Acid 96 ISample Extraction Purification Tube Kit, Sivakalelwa kukuba umsebenzi onomdla, owophula umhlaba kunye nabasebenzi abaqeqeshwe kakuhle unokudala unxulumano oluhle kakhulu noluluncedo ngokufanayo kunye nawe ngokukhawuleza.Qiniseka ukuba uziva ukhululekile ngokupheleleyo ukuqhagamshelana nathi ngeenkcukacha ezithe vetshe.
Sikholelwa ukuba intsebenziswano ende yokubonisa isisiphumo somgangatho ophezulu, iinkonzo ezixabisa ukongezwa, amava anempumelelo kunye noqhagamshelwano lobuquI-China Nucleic Acid Extraction Kit (rna-DNA) kunye ne-96 Nucleic Acid, Ukugxininisa kulawulo olukumgangatho ophezulu wesizukulwana kunye noncedo lwepro yabathengi, ngoku siyile isisombululo sethu sokubonelela abathengi bethu sisebenzisa ukuqalisa ngexabiso lokufumana kunye nasemva kwenkonzo yamava asebenzayo.Ukugcina ubudlelwane obukhoyo kunye nabathengi bethu, nangona kunjalo sivelisa uluhlu lwethu lwezisombululo ngalo lonke ixesha ukwanelisa iimfuno ezintsha kraca kunye nokubambelela kuphuhliso lwangoku lwemarike yaseMalta.Sikulungele ukujongana namaxhala kwaye senze ukuphucula ukuqonda konke okunokwenzeka kurhwebo lwamazwe ngamazwe.

Iinkcukacha

I-50 Preps, yokucoca i-DNA ye-genomic kwigazi elipheleleyo usebenzisa i-<1ml yegazi

I-Buffer DC: Ikhupha iiseli ezibomvu zegazi egazini.

Isithinteli BL1: Nikezela ngemeko yokwetyisa i-enzymatic.

Foregene Protease Plus: Iisampulu zegazi zokwetyiswa ngokweenzymatic kwindawo yeBuffer BL1.

I-Buffer BL2: Yenze i-Foregene Protease Plus kwaye ibonelele ngendawo yokulayisha i-DNA.

I-Buffer PW: Susa ukungcola okufana neprotheni kunye ne-RNA kwi-DNA.

I-Buffer WB1: Susa iiyoni zetyuwa ezishiyekileyo kwi-DNA.

I-Buffer EB: I-Elute i-DNA kwi-membrane yekholomu yokucoca.

Ikholamu ye-DNA kuphela: I-adsorbs i-DNA ye-genomic kwi-lysate.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.

-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kune-proteases efanayo, kwaye igaya iisampulu zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-40.

-Uncedo: I-centrifugation yenziwa kwiqondo lokushisa, i-4 ° C i-centrifugation okanye i-ethanol precipitation ye-DNA ayifuni.

-Ukhuseleko: Akukho kukhutshwa kwezinto eziphilayo ezisetyenzisiweyo.

-Umgangatho ophezulu: Iziqwenga ze-DNA ze-genomic ezikhutshwe zikhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

Isicelo seKit

Ikulungele ukutsalwa kunye nokucocwa kwe-DNA ye-genomic kwigazi elitsha okanye elinomkhenkce elichasene ne-anticoagulated.

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.

Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.

Isisombululo se-Foregene Protease Plus sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);iya kusebenza ngakumbi kwaye izinzile xa igcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuba igcinwe kwi-4 ° C, khumbula ukuba ungayigcini -20 ° C. Sikholelwa ukuba intsebenziswano ende yokubonisa ngokwenene isiphumo somgangatho ophezulu, iinkonzo ezixabisekileyo, amava aphumeleleyo kunye noqhagamshelwano lobuqu kwi-Factory Outlets ye-Virus Magnetic Bead Indlela ye-Rna-DNA Nucleic Acid , I-Purification ye-Nucleic Acid , i-Purification ye-Purification ye-Purification ye-Sample kunye ne-96 i-Purification i-Purification i-Sampler i-Purification amandla anokudala unxibelelwano oluhle kunye noluluncedo ngokufanayo kunye nawe ngokukhawuleza.Qiniseka ukuba uziva ukhululekile ngokupheleleyo ukuqhagamshelana nathi ngeenkcukacha ezithe vetshe.
Iivenkile zomzimveliso weI-China Nucleic Acid Extraction Kit (rna-DNA) kunye ne-96 Nucleic Acid, Ukugxininisa kulawulo olukumgangatho ophezulu wesizukulwana kunye noncedo lwepro yabathengi, ngoku siyile isisombululo sethu sokubonelela abathengi bethu sisebenzisa ukuqalisa ngexabiso lokufumana kunye nasemva kwenkonzo yamava asebenzayo.Ukugcina ubudlelwane obukhoyo kunye nabathengi bethu, nangona kunjalo sivelisa uluhlu lwethu lwezisombululo ngalo lonke ixesha ukwanelisa iimfuno ezintsha kraca kunye nokubambelela kuphuhliso lwangoku lwemarike yaseMalta.Sikulungele ukujongana namaxhala kwaye senze ukuphucula ukuqonda konke okunokwenzeka kurhwebo lwamazwe ngamazwe.