Sicinga ukuba abathengi bacinga ntoni, ukungxamiseka kokungxamiseka ukwenza izinto ngexesha lomdla womthengi wethiyori, ukuvumela umgangatho ongcono kakhulu, ukucutha iindleko zokusetyenzwa, iintlawulo zisengqiqweni ngakumbi, ziphumelele abathengi abatsha nabaphelelwe lixesha inkxaso kunye noqinisekiso lweChina Umboneleli weGolide weFluorescence Ixesha lokwenyani iRt-PCR yongeza izibonelelo ezifudumeleyo zeReagent kwiNucle yoVavanyo ukusondela kwixesha elizayo.
Sicinga ukuba abathengi bacinga ntoni, ukungxamiseka kokungxamiseka ukwenza ngexesha lomdla wesikhundla somthengi wethiyori, ukuvumela umgangatho ongcono kakhulu, ukuncitshiswa kweendleko zokusetyenzwa, iintlawulo zisengqiqweni ngakumbi, ziphumelele abathengi abatsha nabaphelelwe lixesha inkxaso kunye nokuqinisekiswa.Ikhithi yeReagent ye-PCR yaseTshayina kunye noVavanyo lwe-Rt-PCR, Ngenjongo ye "zero defect".Ukukhathalela okusingqongileyo, kunye nembuyekezo yentlalo, ukukhathalela uxanduva lomqeshwa wezentlalo njengomsebenzi wakhe.Samkela abahlobo abavela kwihlabathi liphela ukuba basindwendwele kwaye basikhokele ukuze sifezekise injongo yokuphumelela kunye.

Iinkcazelo Kit

I-2 × I-PCR yangempela elula^TMUmxube-SYBR obonelelwa yi-Real Time PCR Easy^TM-SYBR Green I kit yinkqubo entsha ye-premix esebenzisa i-SYBR Green I yeReality Time PCR reactions yokukhulisa, enokuphucula kakhulu imveliso ethile kunye nobuntununtunu bokusabela.I-ROX inikezelwa njengedayi yolawulo lwangaphakathi.Ukuqina kwe-fluorescence yale khithi ngama-3-5 amaxesha eemveliso ezifanayo, ezinokuthi zibe novakalelo kwaye zibonise ngokucacileyo ukuxinwa kwe-template ye-DNA ekujoliswe kuyo.

I-2 × I-PCR yangempela elula^TMI-Mix-SYBR iqulethe i-Foregene eshushu-isiqalo esisodwa se-Taq DNA Polymerase.Xa kuthelekiswa nee-enzymes ze-Taq eziqhelekileyo, ineengenelo zokusebenza kwe-amplification ephezulu, isakhono esithile esinamandla sokukhulisa kunye nesantya esisezantsi sokungahambelani.Inokunciphisa ukukhuliswa okungangqaliyo kunye nokuphucula ukuchaneka kwe-PCR.

Iinkcukacha

Iimpawu&izinto eziluncedo

■ Ilula—I-2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza

■ I-Specific-optimized buffer kunye ne-hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.

■ Uvakalelo oluphezulu-lukwazi ukubona iikopi ezisezantsi zetemplate

■ Ukuguquguquka okuhle—okuhambelana nezixhobo ezininzi zePCR zexesha lokwenyani

Isicelo seKit

Uhlalutyo lwe-qPCR

Ukuhamba komsebenzi

Umzobo

Ukugcinwa kunye nobomi beshelufu

Le khithi kufuneka igcinwe kude nokukhanya kwaye kufuneka igcinwe ku -20 ℃.Ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ ixesha elifutshane (iintsuku ezili-10).

Sicinga ukuba abathengi bacinga ntoni, ukungxamiseka kokungxamiseka ukwenza izinto ngexesha lomdla womthengi wethiyori, ukuvumela umgangatho ongcono kakhulu, ukucutha iindleko zokusetyenzwa, iintlawulo zisengqiqweni ngakumbi, ziphumelele abathengi abatsha nabaphelelwe lixesha inkxaso kunye noqinisekiso lweChina Umboneleli weGolide weFluorescence Ixesha lokwenyani iRt-PCR yongeza izibonelelo ezifudumeleyo zeReagent kwiNucle yoVavanyo ukusondela kwixesha elizayo.
China Gold Supplier forIkhithi yeReagent ye-PCR yaseTshayina kunye noVavanyo lwe-Rt-PCR, Ngenjongo ye "zero defect".Ukukhathalela okusingqongileyo, kunye nembuyekezo yentlalo, ukukhathalela uxanduva lomqeshwa wezentlalo njengomsebenzi wakhe.Samkela abahlobo abavela kwihlabathi liphela ukuba basindwendwele kwaye basikhokele ukuze sifezekise injongo yokuphumelela kunye.