China Uluhlu lwamaxabiso aphantsi kwe-China iNucleic Acid yokutsalwa kweReagent yeMagnetic Bead Indlela yeNucleic Acid yokutsalwa kwe-Rna Ukwahlulwa kwe-DNA yokuTshintsha Ikhithi yeLebhu yeReagent uMvelisi kunye noMboneleli |I-Foregene

ULuhlu lwamaxabiso atshiphu eTshayina lweNucleic Acid yokutsalwa kweReagent yeMagnetic Bead Indlela yeNucleic Acid yokutsalwa kweeKits Rna Isolation DNA Purification Kit Reagent

ULuhlu lwamaxabiso atshiphu eTshayina lweNucleic Acid yokutsalwa kweReagent yeMagnetic Bead Method Nucleic Acid Extraction Kits Rna Isolation DNA Purification Kit Lab Reagent Featured Image

Inkcazelo yeKit:

Le khithi isebenzisa itekhnoloji ye-RT PCR yexesha lokwenyani (rRT-PCR) ukufunyaniswa komgangatho we-SARS-CoV-2 (i-ORF1ab gene kunye ne-N gene) ye-nucleic acids kwiisampulu ze-nasopharyngeal okanye ze-oropharyngeal swab, nge-multiplex PCR fluorescent probe method. ukufunyanwa ngokukhawuleza okukhulu.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Sihlala nomgaqo osisiseko "womgangatho ekuqaleni, iinkonzo kuqala, ukuphuculwa okuzinzileyo kunye nokusungula ukuzalisekisa abathengi" kulawulo lwakho kunye "neziphene, izikhalazo zero" njengenjongo yomgangatho.Ukugqibelela inkampani yethu, sinikezela ngempahla ngelixa sisebenzisa umgangatho ophezulu ngexabiso elifanelekileyo lokuthengisa ngexabiso elincinci lexabiso leChina Nucleic Acid Extraction Reagent Magnetic Bead Method Nucleic Acid Extraction Kits Rna Isolation DNA Purification Kit Lab Reagent, Siye saqinisekisa ukuba sinokubonisa izisombululo zoncedo ezikumgangatho ophezulu ngexabiso elifanelekileyo, emva kokujonga kakuhle.Kwaye siya kudala amandla aqaqambileyo.
Sihlala nomgaqo osisiseko "womgangatho ekuqaleni, iinkonzo kuqala, ukuphuculwa okuzinzileyo kunye nokusungula ukuzalisekisa abathengi" kulawulo lwakho kunye "neziphene, izikhalazo zero" njengenjongo yomgangatho.Ukugqibelela inkampani yethu, sinika iimpahla ngelixa sisebenzisa umgangatho ophakamileyo ngexabiso elifanelekileyo lokuthengisaChina Nucleic Acid Reagent, DNA / Rna Extraction Kit, Sikholelwa ukuba ngenkonzo yethu egqwesileyo engaguqukiyo unokufumana okona kusebenza kakuhle kwaye ubize izinto ezincinci kuthi ixesha elide .Sizibophelele ekuboneleleni ngeenkonzo ezingcono kwaye senze ixabiso elithe chatha kubo bonke abathengi bethu.Ndiyathemba ukuba singadala ikamva elingcono kunye.

Iinkcukacha

48 Rxns, 96 Rxns

Hayi.	Icandelo	Isixa				Amacandelo aphambili
Hayi.	Icandelo	48Rxns		96 Rxns		Amacandelo aphambili
1	I-agent yokukhululwa kwe-Nucleic acid	1.4mL / ityhubhu	2 iityhubhu	5.3 mL/ibhotile	1 ibhotile	I-surfactant
2	Umkhuseli we-RNA	27 μL/ityhubhu	ityhubhu e-1	53 μL/ityhubhu	ityhubhu e-1	I-RNase inhibitor
3	Isisombululo sokusabela kwe-SARS-CoV-2	800 μL/ityhubhu	ityhubhu e-1	1600 μL/ityhubhu	ityhubhu e-1	Iprimer, probe, reaction buffer, dNTP
4	Umxube we-enzyme ye-SARS-CoV-2	80 μL/ityhubhu	ityhubhu e-1	160 μL/ityhubhu	ityhubhu e-1	Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV
5	Ulawulo olulungileyo lwe-SARS-CoV-2	100 μL/ityhubhu	ityhubhu e-1	100 μL/ityhubhu	ityhubhu e-1	I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA
6	Ulawulo olubi lwe-SARS-CoV-2	1200 μL/ityhubhu	ityhubhu e-1	1200 μL/ityhubhu	ityhubhu e-1	TE buffer

Iimpawu&izinto eziluncedo

Ngokukhawuleza kwaye kulula.
Gwema ilahleko ye-nucleic acid.
Umda wokubona okuphantsi kunye novakalelo oluphezulu.
Imfuneko yezixhobo eziphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu.

Ukuqonda ukuqonda kunokufikelela kwinqanaba eliphantsi njengeekopi ze-500 / mL).

GCE/mL	Ct ixabiso
GCE/mL	Njini	Uhlobo lweORF1ab
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N / A	N / A
2.00E-01	N / A	N / A
NC	N / A	N / A

Igophe lokukhulisa umgca

igophe lokukhulisa-RT-qPCR

Kusetyenziswa kwanjani

1. Isixhobo esisebenzayo

i-covid-19 ikiti yokubona i-nucleic acid1

2. Inkqubo yokufumanisa

i-covid-19 ikiti yokubona i-nucleic acid2

Isampulu yokusetyenzwa: Isampuli ye-Naopharyngeal okanye i-oropharyngeal swab (inyathelo eli-1 kuphela) (Linda imizuzu eyi-10)

PCR amplificaton(<=55min)

Umsebenzi:

i-covid-19 ikiti yokubona i-nucleic acid4

Ugcino

itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;

yokugcina
ugcino2

Beka ubomi kwishelufa:1 unyaka

Sihlala nomgaqo osisiseko "womgangatho ekuqaleni, iinkonzo kuqala, ukuphuculwa okuzinzileyo kunye nokusungula ukuzalisekisa abathengi" kulawulo lwakho kunye "neziphene, izikhalazo zero" njengenjongo yomgangatho.Ukugqibelela inkampani yethu, sinikezela ngempahla ngelixa sisebenzisa umgangatho ophezulu ngexabiso elifanelekileyo lokuthengisa ngexabiso elincinci lexabiso leChina Nucleic Acid Extraction Reagent Magnetic Bead Method Nucleic Acid Extraction Kits Rna Isolation DNA Purification Kit Lab Reagent, Siye saqinisekisa ukuba sinokubonisa izisombululo zoncedo ezikumgangatho ophezulu ngexabiso elifanelekileyo, emva kokujonga kakuhle.Kwaye siya kudala amandla aqaqambileyo.
Uluhlu lwamaxabiso aphantsi lweChina Nucleic Acid Reagent, DNA/Rna Extraction Kit, Sikholelwa ukuba ngenkonzo yethu egqwesileyo engaguqukiyo unokufumana eyona ntsebenzo intle kwaye ubize izinto ezincinci kuthi kangangexesha elide.Sizibophelele ekuboneleleni ngeenkonzo ezingcono kwaye senze ixabiso elithe chatha kubo bonke abathengi bethu.Ndiyathemba ukuba singadala ikamva elingcono kunye.

Ngaphambili: IXabiso eliPhantsi kakhulu laseTshayina kwi-Stock Vtm Virus yoThutho loMfanekiso wokuQokelelwa kweTube eneSwab

Okulandelayo: Ixesha elifutshane lokukhokela iChina Nanbei Real-Time PCR System for PCR Laboratory

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