Ukwaneliseka kwabaxumi yeyona nto sigxile kuyo.Siphakamisa inqanaba elingaguqukiyo lobuchwephesha, umgangatho ophezulu, intembeko kunye nenkonzo eyilwe ngokufanelekileyo iHipure PCR ePure Mini Kit Buyisela amaqhekeza eDNA phakathi kwe60bp-20kbp, Wamkelekile ukukhangela iincwadi zakho kunye nayo nayiphi na imibuzo oyibuzayo, sinethemba elikhulu lokuba siyakwazi ukuba nethuba lokusebenzisana nawe kwaye siyakwakha umtshato kunye nawe.
Ukwaneliseka kwabaxumi yeyona nto sigxile kuyo.Siphakamisa umgangatho ohambelanayo wobugcisa, umgangatho ophezulu, ukuthembeka kunye nenkonzo yeI-China i-Rna / i-DNA Extraction yokuCocwa kunye ne-Nucleic Acid Isolation, Siye saseka ubudlelwane bexesha elide, obuzinzileyo kunye nobuhle beshishini kunye nabavelisi abaninzi kunye nabathengisi kwihlabathi liphela.Okwangoku, sijonge phambili kwintsebenziswano enkulu ngakumbi nabathengi baphesheya kweelwandle ngokusekwe kwiinzuzo ezifanayo.Qinisekisa ukuba uziva ukhululekile ukuqhagamshelana nathi ngeenkcukacha ezithe kratya.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi

∎ Imfuno yesampulu incinci, kancinane njenge-5mg inyama yesilwanyana inokuvavanywa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: iintlobo ezahlukeneyo zezicubu zezilwanyana.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer AL inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

I-Reagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa kwi-4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃ .

I-Reagent 2 × PCR Easy^TMUmxube ungagcinwa iinyanga ezili-12 kwi -20℃;ukuba isetyenziswa rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (sebenzisa ngaphakathi kweentsuku ezili-10).

Ukwaneliseka kwabaxumi yeyona nto sigxile kuyo.Siphakamisa inqanaba elingaguqukiyo lobuchwephesha, umgangatho ophezulu, intembeko kunye nenkonzo eyilwe ngokufanelekileyo iHipure PCR ePure Mini Kit Buyisela amaqhekeza eDNA phakathi kwe60bp-20kbp, Wamkelekile ukukhangela iincwadi zakho kunye nayo nayiphi na imibuzo oyibuzayo, sinethemba elikhulu lokuba siyakwazi ukuba nethuba lokusebenzisana nawe kwaye siyakwakha umtshato kunye nawe.
Yenziwe kakuhleI-China i-Rna / i-DNA Extraction yokuCocwa kunye ne-Nucleic Acid Isolation, Siye saseka ubudlelwane bexesha elide, obuzinzileyo kunye nobuhle beshishini kunye nabavelisi abaninzi kunye nabathengisi kwihlabathi liphela.Okwangoku, sijonge phambili kwintsebenziswano enkulu ngakumbi nabathengi baphesheya kweelwandle ngokusekwe kwiinzuzo ezifanayo.Qinisekisa ukuba uziva ukhululekile ukuqhagamshelana nathi ngeenkcukacha ezithe kratya.