Injongo yethu iya kuba kukuzalisekisa abathengi bethu ngokunikela ngoncedo lwegolide, ixabiso elimangalisayo kunye nomgangatho ophezulu woMgangatho oPhakamileyo waseTshayina 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit eneMagnetic Bead Method yokuFumana iPCR, Ngoku sineqela elinezakhono kurhwebo lwamazwe ngamazwe.Siza kusombulula ingxaki odibana nayo.Singabonisa iimpahla ozifunayo.Qiniseka ukuba uzive usimahla ukunxibelelana nathi.
Injongo yethu iya kuba kukuzalisekisa abathengi bethu ngokunikela ngoncedo lwegolide, ixabiso elimangalisayo kunye nomgangatho ophezulu weChina DNA Rna Isolation, I-Viral Nucleic Acid Isolation, Inkonzo yangoko nangoko emva kokuthengiswa kwenkonzo ebonelelwa liqela lethu labacebisi bonwabile abathengi bethu.Ulwazi oluneenkcukacha kunye neeparameters ezivela kwintengiso mhlawumbi ziya kuthunyelwa kuwe nakuphi na ukuvuma okubanzi.Iisampulu zasimahla zinokuziswa kwaye inkampani ijonge kwiqumrhu lethu.n Morocco thethwano yamkelekile rhoqo.Ndiyathemba ukufumana imibuzo kukuchwetheza kwaye wakhe intsebenziswano yexesha elide.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Injongo yethu iya kuba kukuzalisekisa abathengi bethu ngokunikela ngoncedo lwegolide, ixabiso elimangalisayo kunye nomgangatho ophezulu woMgangatho oPhakamileyo waseTshayina 96 Well Plate Test DNA/Rna Nucleic Acid Extract Isolation Reagent Kit eneMagnetic Bead Method yokuFumana iPCR, Ngoku sineqela elinezakhono kurhwebo lwamazwe ngamazwe.Siza kusombulula ingxaki odibana nayo.Singabonisa iimpahla ozifunayo.Qiniseka ukuba uzive usimahla ukunxibelelana nathi.
Umgangatho ophezulu weChina DNA Rna Isolation, I-Viral Nucleic Acid Isolation, Inkonzo yangoko nangoko emva kokuthengiswa kwenkonzo ebonelelwa liqela lethu labacebisi bonwabile abathengi bethu.Ulwazi oluneenkcukacha kunye neeparameters ezivela kwintengiso mhlawumbi ziya kuthunyelwa kuwe nakuphi na ukuvuma okubanzi.Iisampulu zasimahla zinokuziswa kwaye inkampani ijonge kwiqumrhu lethu.n Morocco thethwano yamkelekile rhoqo.Ndiyathemba ukufumana imibuzo kukuchwetheza kwaye wakhe intsebenziswano yexesha elide.