Sikholelwa: Ukusungula izinto ezintsha ngumphefumlo nomoya wethu.Umgangatho ophezulu bubomi bethu.Imfuno yomthengi nguThixo wethu kumthombo weFactory China CE/FDA eVunyiweyo yeMaso yeMagnetic Indlela yeDNA / iRna Nucleic Acid Extraction Kits (Amaso kaMagnetic), Qiniseka ukuba unamava asimahla okunxibelelana nathi nangaliphi na ixesha.Siza kukuphendula xa sifumana imibuzo yakho.Nceda uqaphele ukuba iisampulu ziyafumaneka ngaphambi kokuba siqale ishishini lethu.
Sikholelwa: Ukusungula izinto ezintsha ngumphefumlo nomoya wethu.Umgangatho ophezulu bubomi bethu.Imfuno yomthengi nguThixo wethuChina Nucleic Acid, China Nucleic Acid Abavelisi, Ngaphandle kokuqina okuqinileyo kobugcisa, sikwazisa izixhobo eziphambili zokuhlola kunye nokulawula ulawulo olungqongqo.Bonke abasebenzi benkampani yethu bamkela abahlobo abasekhaya nakwamanye amazwe ukuba beze kutyelelo kunye neshishini ngokwesiseko sokulingana kunye nenzuzo efanayo.Ukuba unomdla kuyo nayiphi na into yethu, khumbula ukuba uzive ukhululekile ukuqhagamshelana nathi ngekowuteshini kunye neenkcukacha zemveliso.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sikholelwa: Ukusungula izinto ezintsha ngumphefumlo nomoya wethu.Umgangatho ophezulu bubomi bethu.Imfuno yomthengi nguThixo wethu kumthombo weFactory China CE/FDA eVunyiweyo yeMaso yeMagnetic Indlela yeDNA / iRna Nucleic Acid Extraction Kits (Amaso kaMagnetic), Qiniseka ukuba unamava asimahla okunxibelelana nathi nangaliphi na ixesha.Siza kukuphendula xa sifumana imibuzo yakho.Nceda uqaphele ukuba iisampulu ziyafumaneka ngaphambi kokuba siqale ishishini lethu.
Umthombo wefektriChina Nucleic Acid, China Nucleic Acid Abavelisi, Ngaphandle kokuqina okuqinileyo kobugcisa, sikwazisa izixhobo eziphambili zokuhlola kunye nokulawula ulawulo olungqongqo.Bonke abasebenzi benkampani yethu bamkela abahlobo abasekhaya nakwamanye amazwe ukuba beze kutyelelo kunye neshishini ngokwesiseko sokulingana kunye nenzuzo efanayo.Ukuba unomdla kuyo nayiphi na into yethu, khumbula ukuba uzive ukhululekile ukuqhagamshelana nathi ngekowuteshini kunye neenkcukacha zemveliso.