Ilungu ngalinye elinye elivela kwimveliso yethu ephezulu yokuthengisa imveliso ixabisa iimfuno zabathengi kunye nonxibelelwano lombutho weOEM Supply China Lab Universal Reagents Magnetic Bead Nucleic Acid Extraction Kits Viral Rna Isolation Purification, Ishishini lethu ligxininisa kwizinto ezintsha zokukhuthaza uphuhliso oluzinzileyo lwenkampani, kwaye isenze sibe ngababoneleli abakumgangatho ophezulu basekhaya.
Ilungu ngalinye elinye elivela kwimveliso yethu ephezulu yokuthengisa abasebenzi lixabisa iimfuno zabathengi kunye nonxibelelwano lwentlanganoUkuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, China Universal Rna DNA Reagents, sizimisele ngokupheleleyo ukulawula lonke ikhonkco lonikezelo ukuze sihambise iimpahla ezisemgangathweni ngexabiso elikhuphisanayo ngexesha elifanelekileyo.Siqhubeka nobuchule obuphambili, sikhula ngokudala amaxabiso angaphezulu kubathengi bethu nakuluntu.

Inkcazo

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Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ inyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) .Ilungu ngalinye elivela kwimveliso yethu ephezulu yokuthengisa imveliso ixabisa iimfuno zabathengi kunye nonxibelelwano lombutho we-OEM Supply China Lab Universal Reagents Magnetic Bead Nucleic Acid Extraction Kits kwi-Viral Rna Isolation ukunyanzelisa ukuhlanjululwa kwentsholongwane, ukukhuthaza uphuhliso lwethu lwasekhaya kunye nokukhuthaza ukuhlanjululwa kwentsholongwane. -ababoneleli abasemgangathweni.
Ubonelelo lweOEMChina Universal Rna DNA Reagents, Ukuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, sizimisele ngokupheleleyo ukulawula lonke ikhonkco lonikezelo ukuze sihambise iimpahla ezisemgangathweni ngexabiso elikhuphisanayo ngexesha elifanelekileyo.Siqhubeka nobuchule obuphambili, sikhula ngokudala amaxabiso angaphezulu kubathengi bethu nakuluntu.