Isampulu yasimahla ye-China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2
Inkcazelo yeKit:
◮Ikhithi enenyathelo elinye yenza ukukhutshelwa umva kunye ne-qPCR iimpendulo ezimbini kwityhubhu enye, idinga kuphela ukongeza i-template ye-RNA, iiprimers ezithile ze-PCR kunye ne-RNase-Free ddH.2O.
◮Ikhithi inokukhawuleza kwaye ngokufanelekileyo ihlalutye i-RNA yentsholongwane okanye ilandele i-RNA.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
◮RT-qPCR EasyTM(Inyathelo elinye) -SYBR Green I kit iza ne-ROX yedayi yesalathiso yangaphakathi, engasetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesignali phakathi kwamaqula, ekulungele ukuba abathengi basebenzise kwiimodeli ezahlukeneyo zezixhobo ze-PCR zobungakanani.
Sikholelwa: Ukusungula izinto ezintsha ngumphefumlo nomoya wethu.Umgangatho ophakamileyo bubomi bethu.Umthengi kufuneka abe nguThixo wethu kwisampulu yasimahla ye-China High Sensitivity DNA/Rna One-Step Probe Rt-QpcrI-Kit V2, Ngaphakathi kwamanyathelo ethu, sele sineevenkile ezininzi e-China kwaye izisombululo zethu ziphumelele indumiso evela kubathengi kwihlabathi lonke.Wamkelekile abathengi abatsha nabaphelelwe lixesha ukuba baqhagamshelane nathi kubudlelwane beshishini elide elide.
Sikholelwa: Ukusungula izinto ezintsha ngumphefumlo nomoya wethu.Umgangatho ophakamileyo bubomi bethu.Umthengi kufuneka abe nguThixo wethuChina Taq DNA Polymerase, Qpcr, Umsebenzi onzima ukugcina uqhubela phambili, ukuveliswa kwezinto ezintsha kwishishini, yenza yonke imizamo kwishishini lokuqala.Sizama konke okusemandleni ethu ukwakha imodeli yolawulo lwezenzululwazi, ukufunda ulwazi oluninzi lobuchwephesha, ukuphuhlisa izixhobo zemveliso eziphambili kunye nenkqubo yokuvelisa, ukwenza iimveliso ezisemgangathweni zomnxeba wokuqala, ixabiso elifanelekileyo, umgangatho ophezulu wenkonzo, ukuhanjiswa ngokukhawuleza, ukukunika ukudala ixabiso elitsha.
Iinkcukacha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Amacandelo ekhithi
| Icandelo I | Isithinteli P1 |
| Isithinteli P2 | |
| I-6×I-DNA yokuLayisha i-Buffer | |
| Icandelo II | 2× Igqabi PCR EasyTMUmxube(UNG) |
Iimpawu&izinto eziluncedo
■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi
■ imathiriyeli encinci
■ I-Simple-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya
■ 2 × Ukuxuba, ukunciphisa iimpazamo zesampulu zokulayisha kunye nexesha lokulungiselela inkqubo yokusabela
■ Ulungiselelo olukhawulezayo lwetemplate lunokugqityezelwa kwimizuzu eli-10, ukusabela kwe-PCR kunokugqityezelwa ubuncinane kwimizuzu engama-50.
■ Ukusabela okuphezulu kwe-throughput-lysis kunokugqitywa kwi-96-well PCR plate
Iiparamitha zeKit
Isicelo: ukuchongwa okuguqulwe ngokwemfuza, i-genotyping, njl.
Isampulu: igqabi lesityalo
Ubungakanani: Ububanzi 2-3mm (indlela ngqo), ububanzi 5-7mm (indlela lysis)
Uluhlu lokuchongwa: iqhekeza ekujoliswe kulo ≤1kb
Ukuhamba komsebenzi
Electropherogram
Iiveki ezimbini ze-PCR Mix iziphumo zokufumanisa phantsi kwemeko ye-PCR yokukhulisa imveliso ye-contamination.Sikholelwa kuyo: Ukwenziwa kwezinto ezintsha ngumphefumlo wethu nomoya.Umgangatho ophakamileyo bubomi bethu.Umthengi kufuneka abe noThixo wethu wesampuli yaMahala ye-China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, Ngaphakathi kwamanyathelo ethu, sele sineevenkile ezininzi e-China kwaye izisombululo zethu ziphumelele indumiso evela kubathengi emhlabeni wonke.Wamkelekile abathengi abatsha nabaphelelwe lixesha ukuba baqhagamshelane nathi kubudlelwane beshishini elide elide.
Isampulu yasimahla yeChina Taq DNA Polymerase, Qpcr, Umsebenzi onzima ukugcina usenza inkqubela, ukuveliswa kwezinto ezintsha kushishino, yenza zonke iinzame kwishishini lodidi lokuqala.Sizama konke okusemandleni ethu ukwakha imodeli yolawulo lwezenzululwazi, ukufunda ulwazi oluninzi lobuchwephesha, ukuphuhlisa izixhobo zemveliso eziphambili kunye nenkqubo yokuvelisa, ukwenza iimveliso ezisemgangathweni zomnxeba wokuqala, ixabiso elifanelekileyo, umgangatho ophezulu wenkonzo, ukuhanjiswa ngokukhawuleza, ukukunika ukudala ixabiso elitsha.
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