Ngenkqubo epheleleyo yolawulo olugqwesileyo lwezenzululwazi, umgangatho omkhulu kunye nokholo olubalaseleyo, sifumana ukuma okuhle kakhulu kwaye sahlala kweli shishini iminyaka eyi-18 yeFactory yaseChina yeViral DNA/Rna Pre-Package Extraction Kit ye-96automated Nucleic Acid Extraction Laboratory Instrument, sinokusombulula iingxaki zethu zabathengi ngokukhawuleza kwaye senze inzuzo kumthengi wethu.Ukuba ufuna inkampani ebalaseleyo kunye nomgangatho ophezulu , pls sikhethe , enkosi !
Ngenkqubo epheleleyo yolawulo olugqwesileyo lwezenzululwazi, umgangatho omkhulu kunye nokholo olugqwesileyo, sifumana ukuma okuhle kakhulu kwaye sahlala kweli shishiniChina Extraction Reagent, Viral Dna Rna Extraction Kit, Sinokuhlangabezana neemfuno ezahlukeneyo zabathengi ekhaya nakwamanye amazwe.Samkela abathengi abatsha nabadala ukuba beze kuthethathethwano kunye nathi.Ukwaneliseka kwakho yinkuthazo yethu!Masisebenze kunye ukuze sibhale isahluko esitsha esiqaqambileyo!

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Ngenkqubo epheleleyo yolawulo olugqwesileyo lwezenzululwazi, umgangatho omkhulu kunye nokholo olubalaseleyo, sifumana ukuma okuhle kakhulu kwaye sahlala kweli shishini iminyaka eyi-18 yeFactory yaseChina yeViral DNA/Rna Pre-Package Extraction Kit ye-96automated Nucleic Acid Extraction Laboratory Instrument, sinokusombulula iingxaki zethu zabathengi ngokukhawuleza kwaye senze inzuzo kumthengi wethu.Ukuba ufuna inkampani ebalaseleyo kunye nomgangatho ophezulu , pls sikhethe , enkosi !
Iminyaka eyi-18 yeFactoryChina Extraction Reagent, I-Viral DNA Rna Extraction Kit, Sinokuhlangabezana neemfuno ezahlukeneyo zabathengi ekhaya nakwamanye amazwe.Samkela abathengi abatsha nabadala ukuba beze kuthethathethwano kunye nathi.Ukwaneliseka kwakho yinkuthazo yethu!Masisebenze kunye ukuze sibhale isahluko esitsha esiqaqambileyo!