Sizibophelele ekunikeni lula, ukonga ixesha kunye nokugcina imali enye yokuyeka ukuthenga inkxaso kubathengi kwikhithi yokutsalwa kwe-nucleic acid, Samkela ngokunyanisekileyo abathengi baphesheya ukuba babhekiselele kwintsebenziswano yexesha elide kunye nenkqubela phambili.
Sizibophelele ekunikezeni lula, ukonga ixesha kunye nokugcina imali enye-stop inkxaso yokuthenga abathengiUvavanyo lwe-Nucleic Acid, Ngokuqinisekileyo, ixabiso elikhuphisanayo, iphakheji efanelekileyo kunye nokuhanjiswa ngexesha liya kuqinisekiswa ngokwemfuno zabathengi.Sithemba ngokunyanisekileyo ukwakha ubudlelwane beshishini kunye nawe ngesiseko senzuzo efanayo kunye nenzuzo kwikamva elikufuphi kakhulu.Wamkelekile ngokufudumeleyo ukuba uqhagamshelane nathi kwaye ube ngabaququzeleli bethu ngokuthe ngqo.

Iinkcukacha

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho).

Sizibophelele ekunikezeni lula, ukonga ixesha kunye nokugcina imali enye yokuyeka ukuthenga inkxaso yabathengi beOEM/ODM China China Nokonden Mobile ThermometricUvavanyo lwe-Nucleic AcidBooth, Samkela ngokunyanisekileyo abathengi baphesheya ukuba babhekiselele kwintsebenziswano yexesha elide kunye nenkqubela phambili.
OEM / ODM China China Thermometric,Uvavanyo lwe-Nucleic Acid, Ngokuqinisekileyo, ixabiso elikhuphisanayo, iphakheji efanelekileyo kunye nokuhanjiswa ngexesha liya kuqinisekiswa ngokwemfuno zabathengi.Sithemba ngokunyanisekileyo ukwakha ubudlelwane beshishini kunye nawe ngesiseko senzuzo efanayo kunye nenzuzo kwikamva elikufuphi kakhulu.Wamkelekile ngokufudumeleyo ukuba uqhagamshelane nathi kwaye ube ngabaququzeleli bethu ngokuthe ngqo.