Sihlala siwenza rhoqo umoya wethu “wokusungula okuzisa uphuhliso, umgangatho ophezulu wokwenza ukuphila okuthile, intengiso yolawulo kunye nenzuzo yokuthengisa, Amanqaku etyala atsala abathengi kwiHigh Performance China Fully Automated Medical Equipment Magnetic Nucleic Acid DNA Rna Extraction 32-Isiphumo, Nceda usithumelele iinkcukacha zakho okanye ukhululeke ukudibana nathi okanye unemibuzo.
Sihlala siwuphumeza umoya wethu wokuba “Utshintsho oluzisa uphuhliso, Umgangatho ophezulu wokwenza ukutya okuthile, intengiso yoLawulo kunye nenzuzo yokuthengisa, Amanqaku etyala atsala abathengiI-Automatic Nucleic Acid Purification Extractor, China Nucleic Acid Extractor, Ukuze senze abantu abaninzi bazi iimveliso zethu kunye nokwandisa imarike yethu, siye sanikela ingqalelo eninzi kwizinto ezintsha zobugcisa kunye nokuphucula, kunye nokutshintshwa kwezixhobo.Okokugqibela, sikwanika ingqwalasela enkulu ekuqeqesheni abasebenzi bethu bolawulo, amagcisa nabasebenzi ngendlela ecwangcisiweyo.

Iinkcukacha

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Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides okanye umxholo we-polyphenols.Inika ikholomu ye-DNA-Cleaning enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Ngokukodwa ifanele ukucocwa kwe-RNA kwiisampuli zezityalo ze-polysaccharides kunye ne-polyphenols.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
∎ Isampulu: Izihlunu zezityalo ezitsha okanye ezikhenkcezisiweyo zeepolysaccharides neepolyphenols
∎ Umthamo: 50mg izicubu zezityalo
■ Ubuninzi bomthamo wokubopha i-RNA yekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA epheleleyo kwiisampuli zezicubu zezityalo ezitsha okanye ezikhenkcezisiweyo (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye nomxholo ophezulu we-polysaccharide kunye ne-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ugcino kunye nobomi beShelf

Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
I-Buffer PSL1 inokubekwa ku-4℃ kwinyanga e-1 emva kokudibanisa i-β-mercaptoethanol (kuyacetyiswa ukuba uyidibanise ngexesha elifanayo lovavanyo).

Sihlala siwenza rhoqo umoya wethu “wokusungula okuzisa uphuhliso, umgangatho ophezulu wokwenza ukuphila okuthile, intengiso yolawulo kunye nenzuzo yokuthengisa, Amanqaku etyala atsala abathengi kwiHigh Performance China Fully Automated Medical Equipment Magnetic Nucleic Acid DNA Rna Extraction 32-Isiphumo, Nceda usithumelele iinkcukacha zakho okanye ukhululeke ukudibana nathi okanye unemibuzo.
Ukwenza okuphezuluChina Nucleic Acid Extractor, I-Automatic Nucleic Acid Purification Extractor, Ukuze senze abantu abaninzi bazi iimveliso zethu kunye nokwandisa imarike yethu, siye sanikela ingqalelo eninzi kwizinto ezintsha zobugcisa kunye nokuphucula, kunye nokutshintshwa kwezixhobo.Okokugqibela, sikwanika ingqwalasela enkulu ekuqeqesheni abasebenzi bethu bolawulo, amagcisa nabasebenzi ngendlela ecwangcisiweyo.