Ukuhlangabezana nolwaneliseko olulindelwe ngabathengi, sineqela lethu elomeleleyo lokubonelela ngeyona nkxaso iphezulu-yonke ebandakanya ukuthengisa, ingeniso, ukuza, imveliso, ulawulo olugqwesileyo, ukupakisha, indawo yokugcina iimpahla kunye nolungiselelo lweeMveliso eziNtsha eziTshayo zaseChina iPlastiki yePlastiki yeMagnetic Bead yeUniversal Nucleic Acid Extraction Rapid Kit, ngoku sijonge phambili ngakumbi kubathengi.Qinisekisa ukuba uziva kungekho ndleko ukuqhagamshelana nathi ngolwazi oluthe kratya.
Ukuhlangabezana nolwaneliseko olulindelwe ngabathengi, sineqela lethu elomeleleyo lokubonelela ngeyona nkxaso ibalaseleyo equka ukuthengisa, ingeniso, ukuza nayo, imveliso, ulawulo olugqwesileyo, ukupakisha, ugcino kunye nolungiselelo lwempahla.China Universal Nucleic Acid Extraction, IPlastiki yeNucleic Acid Extraction, Ngoku ukhuphiswano kule ntsimi lubukhali kakhulu;kodwa siseza kubonelela ngowona mgangatho uphezulu, ixabiso elifanelekileyo kunye neyona nkonzo inolwazelelelo kwiinzame zokufezekisa injongo yokuphumelela.“Yitshintshe ube ngcono!”sisiqubulo sethu, esithetha ukuthi “Ihlabathi elilunge ngakumbi liphambi kwethu, ngoko masilonwabele!”Guqula ube ngcono!Sele ulungele?

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa kwi-4 ℃ inyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho). Ukuhlangabezana nokwaneliseka okulindelwe ngabathengi, sineqela lethu elomeleleyo lokunikela ngenkxaso yethu egqwesileyo equka ukuthengisa, ingeniso, ukuza kunye, imveliso, ukulawula okugqwesileyo, ukupakisha, i-warehousing yePlastiki ye-Plastiki eNtsha ye-Acthousing ye-Acthousing ye-Acthousing ye-China kunye ne-Logistics ye-Plasic I-Extraction Rapid Kit, Okwangoku, sijonge phambili kwintsebenziswano enkulu ngakumbi nabathengi baphesheya kweelwandle ngokusekwe kwiinzuzo ezifanayo.Qinisekisa ukuba uziva kungekho ndleko ukuqhagamshelana nathi ngolwazi oluthe kratya.
Iimveliso eziNtsha ezishushuChina Universal Nucleic Acid Extraction, IPlastiki yeNucleic Acid Extraction, Ngoku ukhuphiswano kule ntsimi lubukhali kakhulu;kodwa siseza kubonelela ngowona mgangatho uphezulu, ixabiso elifanelekileyo kunye neyona nkonzo inolwazelelelo kwiinzame zokufezekisa injongo yokuphumelela.“Yitshintshe ube ngcono!”sisiqubulo sethu, esithetha ukuthi “Ihlabathi elilunge ngakumbi liphambi kwethu, ngoko masilonwabele!”Guqula ube ngcono!Sele ulungele?