Ibambelela kumgaqo othi "Inyanisekile, ikhuthele, inomdla, ivelisa izinto ezintsha" ukuphuhlisa iimveliso ezintsha rhoqo.Ijonga abathengi, impumelelo njengempumelelo yayo.Masiphuhlise ikamva elinempumelelo kwikholamu ye-Wholesale spin Indlela ye-Nucleic Acid Extraction Reagent Bottle Kits, Singakubonisa elinye lawona maxabiso athengisayo akhuphisanayo kunye nomgangatho olungileyo, kuba sithe saBafaneleka ngakumbi!Ngoko akufanele ungathandabuzi ukusitsalela umnxeba.
Ibambelela kumgaqo othi "Inyanisekile, ikhuthele, inomdla, ivelisa izinto ezintsha" ukuphuhlisa iimveliso ezintsha rhoqo.Ijonga abathengi, impumelelo njengempumelelo yayo.Masiphuhlise ikamva elinempumelelo ngesandla sibambeneChina Nucleic Acid Extraction Kit (spin ikholamu), Uvavanyo lwe-Nucleic Acid, Ukuseka ubudlelwane bexesha elide kunye nokuphumelela-win ishishini kunye nabo bonke abathengi bethu, yabelana ngempumelelo kwaye ujabulele ulonwabo lokusasaza iimpahla zethu kwihlabathi kunye.Sithembe kwaye uya kuzuza ngaphezulu.Nceda uzive ukhululekile ukuqhagamshelana nathi ngolwazi oluthe kratya, siyakuqinisekisa ngengqalelo yethu ngamaxesha onke.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) .Inamathela kwi-tenet "Ukunyaniseka, ukukhuthala, ukungenisa, ukusungula" ukuphuhlisa iimveliso ezintsha rhoqo.Ijonga abathengi, impumelelo njengempumelelo yayo.Masiphuhlise ikamva elinempumelelo esandleni kwi-Wholesale ye-Wholesale yase-China ye-Magnetic Beads Method ye-Nucleic Acid Detection Testing Extraction Diagnostic Reagent Bottle Kits, Singakubonisa elinye lawona maxabiso athengisayo akhuphisanayo kunye nomgangatho olungileyo, kuba sithe saBafaneleka kakhulu!Ngoko akufanele ungathandabuzi ukusitsalela umnxeba.
Ixabiso leWholesale yaseChina iNucleic Acid Extraction Kit (amaso kazibuthe) ,Uvavanyo lwe-Nucleic Acid, Ukuseka ubudlelwane bexesha elide kunye nokuphumelela-win ishishini kunye nabo bonke abathengi bethu, yabelana ngempumelelo kwaye ujabulele ulonwabo lokusasaza iimpahla zethu kwihlabathi kunye.Sithembe kwaye uya kuzuza ngaphezulu.Nceda uzive ukhululekile ukuqhagamshelana nathi ngolwazi oluthe kratya, siyakuqinisekisa ngengqalelo yethu ngamaxesha onke.