Plant Total RNA Isolation Plus Kit
Inkcazelo yeKit:
Susa ngokufanelekileyo i-DNA usebenzisa i-DNA-Cleaning Column
Susa i-DNA ngaphandle kokongeza i-DNase
Elula-yonke imisebenzi igqitywe kwiqondo lokushisa
Ukukhawuleza-ukusebenza kunokugqitywa kwimizuzu engama-30
Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
Iinkcukacha
50 Preps, 200 Preps
Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nekhwalithi ephezulu ye-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides okanye umxholo we-polyphenols.Inika i-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.
Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.
Amacandelo ekhithi
| Buffer PSL1, Buffer PS, Buffer PSL2 |
| Isithinteli PRW1, Isithinteli PRW2 |
| RNase-Free ddH2O, iKholamu yokucoca iDNA |
| Ikholamu ye-RNA Kuphela |
Iimpawu&izinto eziluncedo
■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Ngokukodwa ifanele ukucocwa kwe-RNA kwiisampuli zezityalo ze-polysaccharides kunye ne-polyphenols.
∎ IKholamu yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.
Iiparamitha zemveliso
■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
∎ Isampulu: Izihlunu zezityalo ezitsha okanye ezikhenkcezisiweyo zeepolysaccharides neepolyphenols
∎ Umthamo: 50mg izicubu zezityalo
■ Ubuninzi bomthamo wokubopha i-RNA yekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl
Isicelo seKit
Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zezityalo ezitsha okanye ezikhenkcezisiweyo (ingakumbi izicubu zamagqabi esityalo esitsha) ezinepolysaccharide ephezulu kunye nomxholo wepolyphenol.
Ukuhamba komsebenzi
Umzobo
Isityalo esipheleleyo se-RNA Isolation Kit Plus iqhutywe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati
Ugcino kunye nobomi beShelufu
Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
I-Buffer PSL1 inokubekwa ku-4℃ kwinyanga e-1 emva kokongeza i-β-mercaptoethanol (kuyacetyiswa ukuba yongezwe ngexesha elinye lovavanyo).
