Sicinga ukuba abathengi bacinga ntoni, ukungxamiseka kokungxamiseka ukwenza izinto ezivela kumthengi womgaqo-siseko, ukuvumela umgangatho ophezulu, ukuhla kweendleko zokucwangcisa, uluhlu lwamaxabiso lusengqiqweni ngakumbi, luphumelele amathemba amatsha kunye nabadala benkxaso kunye noqinisekiso lwe-Wholesale Discount China Rna Nucleic Acid Extraction/Reagent yokuCoca ngeVirus Sampling Swab Kit (Indlela ye-Magnetic Bead) kunye ne-CE kunye ne-ISO, Siza kwenza iinzame ezongeziweyo zokunceda abathengi basekhaya nabamazwe ngamazwe, kwaye sivelise inzuzo efanayo kunye nokuphumelela ngokubambisana phakathi kwethu.silindele ngolangazelelo intsebenziswano yakho ngokunyanisekileyo.
Sicinga ukuba abathengi bacinga ntoni, ukungxamiseka kokungxamiseka kokuthatha amanyathelo kwizinto ezinomdla womthengi womgaqo-siseko, ukuvumela umgangatho ophezulu ophezulu, ukuhla kweendleko zokusetyenzwa, uluhlu lwamaxabiso lusengqiqweni ngakumbi, luphumelele amathemba amatsha kunye nabadala inkxaso kunye nokuqinisekiswa.China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Siyazizukisa njengenkampani equka iqela elomeleleyo leengcali ezivelisa izinto ezintsha nezinamava kurhwebo lwamazwe ngamazwe, uphuhliso lweshishini kunye nokuphuculwa kwemveliso.Ngaphezu koko, inkampani ihlala iyingqayizivele phakathi kwabakhuphisana nabo ngenxa yomgangatho ophezulu womgangatho kwimveliso, kunye nokusebenza kwayo kunye nokuguquguquka kwinkxaso yoshishino.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sicinga ukuba abathengi bacinga ntoni, ungxamiseko longxamiseko lokuthatha amanyathelo kwizinto ezinomdla womthengi womgaqo-siseko, ukuvumela umgangatho ophezulu ophezulu, ukuhla kweendleko zokusetyenzwa, uluhlu lwamaxabiso lusengqiqweni ngakumbi, luphumelele amathemba amatsha kunye nabadala benkxaso kunye noqinisekiso lweDiscount ye-Wholesale yaseChina Rna Nucleic Acid Extraction/Reagent yokuCoca ngeVirus Sampling, iSampuli yeSampuli eyongeziweyo, yandisiwe iSampuli yeSampuli ye-CE ukuya kwi-Method Swab kunye ne-Metho ye-Cead id abathengi basekhaya nabamazwe ngamazwe, kwaye uvelise inzuzo efanayo kunye ne-win-win ubudlelwane phakathi kwethu.silindele ngolangazelelo intsebenziswano yakho ngokunyanisekileyo.
Isaphulelo kwiholeyiliChina Nucleic Acid Extraction Reagent, iMagnetic Bead Method, Siyazizukisa njengenkampani equka iqela elomeleleyo leengcali ezivelisa izinto ezintsha nezinamava kurhwebo lwamazwe ngamazwe, uphuhliso lweshishini kunye nokuphuculwa kwemveliso.Ngaphezu koko, inkampani ihlala iyingqayizivele phakathi kwabakhuphisana nabo ngenxa yomgangatho ophezulu womgangatho kwimveliso, kunye nokusebenza kwayo kunye nokuguquguquka kwinkxaso yoshishino.