I-DNA-Only Column yikholomu ekhethekileyo ye-silica membrane adsorption, edityaniswe ne-Foregene's reagents ye-formula eyodwa, inokubopha ngokufanelekileyo kwaye ibophe ngokukodwa i-DNA, kwaye inokususa i-RNA, iiprotheni ezingcolileyo, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba elikhulu.Inkqubo yovavanyo inokususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase, kwaye inqande ilabhoratri ukuba ingangcoliswa yi-RNase yangaphandle.

KwiKholamu ye-DNA-Only, i-plasmid DNA inokubotshwa kwi-membrane ye-silica phantsi kweemeko zetyuwa ephezulu, kwaye ke i-DNA ikhutshwe kwi-silicon matrix kunye ne-buffer yetyuwa ephantsi okanye amanzi.I-DNA efunyenweyo inokusetyenziselwa imifuniselo yebhayoloji yemolekyuli enje ngothintelo lokwetyisa, iPCR, ulandelelwano, ukwakhiwa kwethala leencwadi, ukuguqulwa kwebhaktiriya, kunye nokudluliselwa.

Iingenelo

Akukho mfuneko yokongeza i-RNase ukususa i-RNA kwi-DNA ye-genomic, ukuthintela ilabhoratri ukuba ingangcoliswa yi-RNase yangaphandle.

Isicelo

Ukukhutshwa kwe-DNA kwiisampulu ezinje ngeeplasmids, izilwanyana, iiseli, izityalo, iintsholongwane, amanzi, ilindle, umhlaba, kunye ne-buccal swabs.