ISerum(Plasma) miRNA Isolation Kit
Inkcazelo yeKit:
◆Isethi epheleleyo yekhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
◆Ikholomu yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukwenzela ukuba ikiti inokususa ukungcoliswa kwe-DNA ye-genomic ngaphandle kokongeza i-DNase eyongezelelweyo.
◆Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
◆Isantya esikhawulezayo: Umsebenzi ulula kwaye unokugqitywa kwimizuzu engama-30.
◆Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
◆Umgangatho ophezulu: Iziqwenga ze-RNA ezikhutshiweyo zinokucoceka okuphezulu, azinaprotheyini kunye nobunye ukungcola, kwaye zinokuhlangabezana nezicelo ezahlukeneyo zokulinga.
Iinkcazelo
Le khithi isebenzisa i-spin ikholamu kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ngokufanelekileyo amaqhekeza amancinci e-miRNA ngaphantsi kwe-100nt kwi-serum kunye neplasma.Ikiti ibonelela nge-DNA-Cleaning Column, enokuthi ihlukanise ngokulula i-supernatant kunye ne-tissue lysate kunye ne-adsorb kwaye isuse i-DNA kwisampuli, elula kwaye igcina ixesha;IKholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA, kwaye ngefomula ekhethekileyo, ingasetyenziswa ngexesha elinye.Yenza inani elikhulu leesampuli.
Yonke inkqubo i-RNase-Free, ukuze i-miRNA ehlanjululweyo ingathotywanga;I-Buffer miBRW1 kunye ne-Buffer miBRW2 inkqubo yokuhlamba i-buffer yenza i-RNA ifumaneke ngaphandle kweprotheyini, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.
Iinkcukacha
5T, 50T, 100T, 200T
Amacandelo ekhithi
| ISerum/Plasma miRNA Isolation Kit | ||||
| Imixholo yekhithi | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
| 5 T | 50 T | I-100T | 200T | |
| Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
| Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
| Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| IKholam ye-RNA kuphela | 5 amaqhekeza | 50amaqhekeza | I-100amaqhekeza | 200amaqhekeza |
| Ikholamu yokucoca iDNA | 5 amaqhekeza | 50amaqhekeza | I-100amaqhekeza | 200amaqhekeza |
| IFU | 1 iqhekeza | 1 iqhekeza | 1 iqhekeza | 1 iqhekeza |
Iimpawu&izinto eziluncedo
◆Isethi epheleleyo yekhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
◆Ikholomu yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukwenzela ukuba ikiti inokususa ukungcoliswa kwe-DNA ye-genomic ngaphandle kokongeza i-DNase eyongezelelweyo.
◆Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
◆Isantya esikhawulezayo: Umsebenzi ulula kwaye unokugqitywa kwimizuzu engama-30.
◆Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
◆Umgangatho ophezulu: Iziqwenga ze-RNA ezikhutshiweyo zinokucoceka okuphezulu, azinaprotheyini kunye nobunye ukungcola, kwaye zinokuhlangabezana nezicelo ezahlukeneyo zokulinga.
Iiparamitha zeKit
Isicelo seKit
Ifanelekile ukutsalwa kunye nokucocwa kwe-miRNA kwisilwanyana kunye neserum yabantu kunye neplasma.
MiRNA Isicelo
I-miRNA ihlanjululwe yi-Serum / Plasma miRNA Isolation Kit ingasetyenziselwa kwiimvavanyo ezahlukeneyo ze-molecular ezantsi, ezifana ne-RT-PCR, i-Real Time RT-PCR, i-Northern Blot, uhlalutyo lwe-chip, njl.
Ukuhamba komsebenzi
Iimeko zokugcina
Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa(15-25°C).Iukuba kufuneka igcinwe ixesha elide,ndiyacelaigcinwe ku-2-8°C.
