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I-ForeDirect RT-qPCR Kit
◮Kwi-RNA yokukhulisa ixesha lokwenyani ngqo ukusuka kwingqokelela ye-swab ngaphandle kweenkqubo zokucoca i-RNA.Le khithi igqiba imijikelo ye-qRT-PCR kwiyure enye.Umxube we-enzyme ngumxube ophuculweyo we-Reverse Transcriptase, i-Hot-Start Taq DNA polymerase, i-RNase inhibitor.I-Reaction Buffer iqulathe onke amacandelo afunekayo, kubandakanywa amacandelo esikhuseli esiphuculweyo, uMg2+, i-dUTP, kunye ne-dNTPs.
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FFPE DNA Isolation Kit DNA Extraction Ikhithi yokuCoca kwiiSampuli zeFFPE
Ngokukhawuleza coca i-DNA ye-genomic ekumgangatho ophezulu ukusuka kwizihlunu ezifakwe kwiparafini ezifana namacandelo eparafini kunye neebhloko zeparafini.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-PCR yokucoca iKhiti ye-PCR yokuCoca-up System ye-Ultra-pure DNA
Ngokukhawuleza uhlambulule kwaye ufumane amaqhekeza e-DNA akumgangatho ophezulu kwinkqubo yePCR;ukuba ufuna ukwahlula kwaye ufumane amaqhekeza athile eDNA, nceda ukhethe ikhithi yokubuyisela ijeli.
◮ Uluhlu olubanzi lokuchacha kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-60bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮ Ukusebenza okuphezulu kokubuyisela: Ukusebenza kokubuyisela ngokuqhelekileyo kungaphezulu kwe-80%, kwaye iphakamileyo inokufikelela ngaphezu kwe-95%.
◮ Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15 kwaye i-primer dimer inokususwa ngokuthe ngqo ngaphandle kokubuyiswa kwe-gel.
◮ Safety:Akukho kutsalwa kwe-organic reagent efunekayo.
◮ Umgangatho ophezulu: Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana novavanyo olwahlukeneyo olulandelayo.
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Iarhente yokukhupha iSampuli
◮Kulula ukusebenza:isampuli lysis ngelixa ingqokelela isampuli
◮Khawuleza kwaye lufanelekile:Akukho mfuneko yokutsalwa kwe-nucleic acid, ilungele ukuchongwa kwesikali esikhulu
◮ Umda obanzi wesicelo:I-PCR ngokuthe ngqo emva kokuqokelela isampulu
◮Ikhuselekile kwaye ithembekile:Yenza intsholongwane ngokukhawuleza ingasebenzi, qinisekisa ukhuseleko ukuya kuthi ga kwinqanaba elikhulu
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I-RT-PCR Easyᵀᴹ I(Inyathelo elinye)
◮Ikiti yenyathelo elinye yenza ukuba uguqulelo loguqulelo kunye nePCR luqhutywe kwityhubhu enye.Ifuna kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH2O.
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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I-RT-PCR Easyᵀᴹ II(Amanyathelo amabini)
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮ Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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Mouse Umsila DNA Mini Kit Genomic DNA Extraction Kits ukusuka Mouse Umsila
Eyona khithi ikhawulezayo yehlabathi yokukhupha umsila wemouse i-genomic DNA, enokukhupha i-DNA ye-genomic ekumgangatho ophezulu kumsila wempuku kwiyure enye.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-Bacterial DNA Isolation Kit I-Bacterial Genomic DNA Extraction Iikhithi zokuCoca
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu evela kwiibhaktheriya kwisigaba sokukhula kwe-logarithmic.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-CCND1/IGH yoMbala oMbini we-Dual Fusion Probe
Ngokomgaqo-siseko we-DNA yokudibanisa okuhambelanayo, i-CCND1 i-orenji-red probe kunye ne-IGH eluhlaza iprobe isetyenziselwe ukuxutywa kunye nokulandelelana kwethagethi ye-DNA kwi-nucleus, kunye nolwazi lwe-gene state kwi-nucleus lwabonwa kwaye lwahlalutywa phantsi kwe-microscope ye-fluorescence.
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I-RB1/1q21 iProbe yoMbala eMabini
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
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I-HER2 / CSP17 iProbe yoMbala oMbini
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
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ETV6 Dual Color Break Apart Probe
Ngokomgaqo-siseko we-DNA odibanisayo wokudibanisa, i-ETV6 i-orenji-red probe kunye ne-ETV6 ye-green probe isetyenziselwe ukuxutywa kunye nolandelelwano lwe-DNA ekujoliswe kuyo kwi-nucleus, kwaye ulwazi lwe-gene state kwi-nucleus lujongwe kwaye luhlalutywe phantsi kwe-microscope ye-fluorescence.
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