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I-Escherichia Coli O157: I-H7 Nucleic Acid Detection Kit (I-PCR Fluorescent Probe Method/Lyophilization)
Ikati.No.FP103
Isetyenziselwa ukukhangela ngokukhawuleza kunye nokuhlolwa kwe-E. coli O157: H7 ekutyeni, ukutya, iisampulu zamanzi kunye neesampuli zokusingqongileyo.
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IViral DNA & RNA Isolation Kit Viral DNA kunye neRNA Extraction yokuLungiselela iiKhithi zokuLungisa
Ikati.No.DR-01011/01012/01013
Ukucocwa kwe-viral DNA/RNA kwi-plasma, i-serum, i-cell-free body fluids, i-cell-culture supernatants.
Yahlula ngokukhawuleza kwaye uhlambulule i-virus ye-DNA okanye i-RNA kwiisampulu ezinjenge-plasma, i-serum, i-cell-free body fluid kunye ne-cell culture supernatant.
Akukho kuthotywa kweRNA.Ikhithi iyonke i-RNase-Free
Elula-yonke imisebenzi igqitywe kwiqondo lokushisa
Ukukhawuleza-ukusebenza kunokugqitywa kwimizuzu engama-20
Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA
Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
Umthamo omkhulu wokusetyenzwa kwesampulu-ukuya kuthi ga kwi-200μl iisampulu zinokucutshungulwa ngexesha ngalinye.
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I-Animal Tissue DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples
Ukukhutshwa ngokukhawuleza kunye nokucocwa kwe-DNA ye-genomic kwimithombo emininzi, njengezicubu zezilwanyana, iiseli, njl.
◮Isantya esiphezulu:umsebenzi ophezulu kunama-proteases afanayo kunye nokugaya iisampuli zezicubu ngokukhawuleza;
◮Lula:inokugqitywa kwimizuzu engama-50.
◮Iluncedo:Yenziwe kwiqondo lokushisa.
◮Ukhuseleko:akukho reagent ephilayo esetyenziswayo.
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Blood DNA Mini Kit
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (<1ml).
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Ngokubanzi iPlasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Ucoca ngokukhawuleza i-plasmid DNA ekumgangatho ophezulu ukusuka kwiibhaktheriya eziguquliweyo zovavanyo lwesiqhelo lwebhayoloji yemolekyuli njengotshintsho kunye nokwetyisa kwe-enzyme.
◮Susa i-RNA ngaphandle kokongeza i-RNAse
◮Iluncedo-i-centrifugation yenziwa kwindawo yobushushu begumbi
◮Ukukhawuleza-umsebenzi unokugqitywa kwimizuzu engama-20
◮Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
◮Ukucoceka okuphezulu—OD260/280≈1.7-1.9
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I-RT Easyᵀᴹ I Master Premix ye-cDNA yomtya wokuqala
- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-25 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
-Inkqubo yokukhuphela umva inozinzo oluphezulu lwe-thermal, ubushushu bokusabela kwenkqubo bunokuphakama ukuya kuma-50 ℃,kwaye inomsebenzi olungileyo wokukhuphela umva.
- Nkqu neesampuli ze-RNA ezingcolileyo (utywala ukuya kwi-45%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.
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I-RT Easy II (kunye ne-gDNase) i-Master Premix yokuqala-strand ye-cDNA synthesis yeXesha lokwenyani PCR ene-gDNase
-Ikhono elisebenzayo lokususa i-gDNA, enokususa i-gDNA kwi-template ngaphakathi kwemizuzu emi-2.
-Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu eyi-15 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-Iitemplates eziyinkimbinkimbi: iitemplates ezinomxholo ophezulu weGC kunye nesakhiwo sesibini esiyinkimbinkimbi sinokuphinda sitshintshwe ngokusebenza okuphezulu.
-I-High-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
I-reverse transcription system inozinzo oluphezulu lwe-thermal, iqondo lokushisa elichanekileyo liyi-42℃, kwaye isenomsebenzi olungileyo wokukhutshelwa umva kwi-50℃.
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Ixesha lokwenyani PCR Easyᵀᴹ-SYBR Green I kit
Lula—2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza
I-Specific-optimized buffer kunye ne-Hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.
Uvakalelo oluphezulu-luyakwazi ukubona iikopi eziphantsi zetemplate
Ukuguquguquka okuhle-kuyahambelana nezixhobo ezininzi zePCR zexesha lokwenyani
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ISilwanyana sisonke se-RNA Isolation Kit Iyonke yeRNA yokutsalwa kunye neeKits zokuCoca kwiiTissues zezilwanyana kunye neSeli
Ngokukhawuleza nangempumelelo ukukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu ezahlukeneyo zezilwanyana.
Akukho mfuneko yakukhathazeka malunga nokonakaliswa kwe-RNA.Yonke inkqubo i-RNase-Free
Susa ngokufanelekileyo i-DNA usebenzisa i-DNA-Cleaning Column
Susa i-DNA ngaphandle kokongeza i-DNase
Elula-yonke imisebenzi igqitywe kwiqondo lokushisa
Ukukhawuleza-ukusebenza kunokugqitywa kwimizuzu engama-30
Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
Ukucoceka okuphezulu-OD260 / 280≈1.8-2.1
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ISeli iyonke yeRNA Isolation Kit Iyonke iRNA Isolation IiKits zokucoca ukusuka kwiseli
Isulungekiswe kakhulu kwaye ikumgangatho ophezulu we-RNA iyonke inokufumaneka kwiiseli ezahlukeneyo zenkcubeko kwimizuzu eli-11.
RNase-Free
Isebenza kubushushu begumbi (15-25℃)
Nge-DNA-Ukucoca iKholam
Isivuno esiphezulu se-RNA
Ukukhawuleza:Gqiba ukutsalwa ngemizuzu eyi-11
Ukhuseleko:Ayikho imichiza ye-Organic
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I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA kwiGazi
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (1-5ml).
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction okanye Purifiction Kit from Buccal Swabs
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwi-buccal swab / iisampulu zeKhadi le-FTA.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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