Ngqo RT-qPCR series
I-PCR ethe ngqo
Yintoni i-PCR eNgqo
I-PCR ethe ngqo yindlela yokukhulisa i-DNA ngokuthe ngqo kwigazi, izicubu zezilwanyana, umsila wempuku, iseli, indlebe yempuku, intlanzi yeqwarhashe, amaqanda entlanzi, amagqabi esityalo, imbewu okanye enye isampulu yebhayoloji yantlandlolo ngaphandle kokwenza ukwahlukaniswa kwe-DNA kunye namanyathelo okucoca.Ubuchule be-PCR obuthe ngqo bunokunciphisa kakhulu ixesha lovavanyo, kunye neendleko kwi-genotyping kunye neeprojekthi zobuninzi obuphezulu.Ikwabonelela ngenketho engcono xa ujongene nemingeni yokukhulisa inani elincinci kakhulu lesampulu apho inyathelo lokucoca linokukhokelela kwilahleko yesampulu.
I-ForEGENE's PCR Mix inoxhathiso oluqinileyo loxinzelelo, ngenxa ye-FOREGENE amandla anelungelo elilodwa lomenzi we-Taq enzyme ( ipatent egunyazisiweyo: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)I-enzyme yayo ye-Taq isebenzisa iteknoloji yokudibanisa i-DNA ukuhlanganisa kwakhona i-DNA ebophelelayo ummandla wesakhiwo se-archaeal nucleic acid ebopha iprotheni kunye ne-Taq DNA polymerase enganyangekiyo ubushushu ukwakha i-template ye-DNA ngokunyuka kobudlelwane.I-Taq ephuculweyo eshushu-isiqalo egcina imisebenzi eyahlukeneyo ye-polymerase ye-DNA yasekuqaleni.Le enzyme ye-Taq inokubophelela kwi-template ye-DNA kwaye iqalise i-DNA synthesis kwi-templates ephantsi yoxinaniso kunye neendawo ezinzima.
Umzekelo, kulula njengokongeza iseli yakho, isityalo (igqabi elincinci, ingcambu okanye imbewu) okanye isampulu yesilwanyana kwi-buffer, kunye nokongeza umxube okhethekileyo oyintloko kunye neeprimers ezithile kwiityhubhu zePCR kwaye uyiqhube ngebhayisekile eshushu.Ukusebenza kweKit kukulungele uhlalutyo lwesampulu enkulu apho ukugcinwa kwexesha kubaluleke kakhulu.
Inkqubo yokusabela kwe-PCR equlethe i-UNG enzyme kunye ne-dUTP ikhethwe ngokweemfuno zovavanyo, ezinokuthi ziphephe ngokufanelekileyo ukungcola okubangelwa yimveliso yokukhulisa i-PCR.
Izinto ezilungileyo zeDPCR echanekileyo
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| I-PCR ethe ngqo | Indlela yemveli (Ukucoca i-RNA+ iCoventional PCR) |
| Ukusetyenziswa kwezinto eziphathekayo | Ukusetyenziswa kwezicubu | Ngaphantsi | Kakhulu |
| Ukulungiselela itemplate | Uhlobo lwezinto | Akukho kusila kuyadingeka | Iisampuli zokugaya |
| Ukusebenza | Uhlobo lwe-tube enye, i-10min | umsebenzi onzima, 1h | |
| Flux | 1-96 | 1-24 | |
| Ukusabela kwePCR | inkqubo yokusabela | Ukulungelelaniswa kwe-2 × PCR umxube | Dntp, MgCl2,10×PCR Buffer,Taq enzyme, |
Aisicelo
01 Ukuchongwa kwe-pathogenic microorganisms
02 Ukuchongwa okuguqulwe ngokwemfuza, ukuchwetheza kwe-genotyping
03 Multiplex PCR / SNP ubhaqo / PCR-RFLP
04Ulandelelwano/ukulinganisa
Series Product
| Uthotho | Igama lemveliso | Iinkcukacha | Inombolo Yekhathalogu | Iimeko zokuGcina |
| Ngqo RT-qPCR Series | 200T | DRT-01011 | Icandelo I 4℃，Icandelo II -20℃ | |
| 1000T | DRT-01012 |
| Uthotho | Igama lemveliso | Iinkcukacha | Inombolo Yekhathalogu | Iimeko zokuGcina |
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Igazi ngqo PCR Series | 200 × 20μl rxns | TP-04111 | -20℃ | |
| 2000 × 20μl rxns | TP-04113 | |||
| 200 × 20μl rxns | TP-04121 | -20℃ | ||
| 2000 × 20μl rxns | TP-04123 | |||
| 200 × 20μl rxns | TP-04211 | -20℃ | ||
| 2000 × 20μl rxns | TP-04213 | |||
| 200 × 20μl rxns | TP-04221 | -20℃ | ||
| 2000 × 20μl rxns | TP-04223 |
| Series | Igama lemveliso | Iinkcukacha | Inombolo Yekhathalogu | Iimeko zokuGcina |
| Animal Direct PCR Series | IKit yePCR yeZilwanyana eziNgqo (65℃ lysis) | 200 × 20μl rxns | TP-01111 | Icandelo I 4℃，Icandelo II -20℃ |
| 2000 × 20μl rxns | TP-01113 | |||
| Animal Tissue Direct PCR Kit(65℃ lysis) -UNG | 200 × 20μl rxns | TP-01121 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-01123 | |||
| Mouse Umsila SuperDirectTMPCR Kit (iqondo lobushushu begumbi) | 200 × 20μl rxns | TP-01311 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-01313 | |||
| IKit yeMouse Tail Direct PCR(65℃ lysis) | 200 × 20μl rxns | TP-01331 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-01333 | |||
| I-Mouse Tail Direct PCR Kit(65℃ lysis) - UNG | 200 × 20μl rxns | TP-01341 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-01343 | |||
| IZebra Fish Direct PCR Kit(65℃ lysis) | 200 × 20μl rxns | TP-01411 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-01413 | |||
| I-Zebra Fish Direct PCR Kit(65℃ lysis) - UNG | 200 × 20μl rxns | TP-01421 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-01423 |
| Uthotho | Igama lemveliso | Iinkcukacha | Inombolo Yekhathalogu | Iimeko zokuGcina |
| Tyala PCR Series Series | 200 × 20μl rxns | TP-02111 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-02113 | |||
| 200 × 20μl rxns | TP-02121 | Icandelo I 4℃，Icandelo II -20℃ | ||
| 2000 × 20μl rxns | TP-02123 | |||
| Tyala Igqabi Direct PCR Plus Kit | 200 × 20μl rxns | TP-02131 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-02133 | |||
| Plant Leaf Direct PCR Plus Kit -UNG | 200 × 20μl rxns | TP-02141 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-02143 | |||
| 200 × 20μl rxns | TP-03111 | Icandelo I 4℃，Icandelo II -20℃ | ||
| 2000 × 20μl rxns | TP-03113 | |||
| 200 × 20μl rxns | TP-03121 | Icandelo I 4℃，Icandelo II -20℃ | ||
| 2000 × 20μl rxns | TP-03123 | |||
| 200 × 20μl rxns | TP-03131 | Icandelo I 4℃，Icandelo II -20℃ | ||
| 2000 × 20μl rxns | TP-03133 | |||
| 200 × 20μl rxns | TP-03141 | Icandelo I 4℃，Icandelo II -20℃ | ||
| 2000 × 20μl rxns | TP-03143 | |||
| Tyala iMbewu ngokuNgqo PCR Plus Kit I(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03151 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-03153 | |||
| Tyala iMbewu ngokuNgqo PCR Plus Kit I-UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03161 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-03163 | |||
| 200 × 20μl rxns | TP-03171 | Icandelo I 4℃，Icandelo II -20℃ | ||
| 2000 × 20μl rxns | TP-03173 | |||
| Tyala iMbewu ngokuNgqo PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03181 | Icandelo I 4℃，Icandelo II -20℃ | |
| 2000 × 20μl rxns | TP-03183 |
