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I-RT-PCR Easyᵀᴹ I(Inyathelo elinye)
◮Ikiti yenyathelo elinye yenza ukuba uguqulelo loguqulelo kunye nePCR luqhutywe kwityhubhu enye.Ifuna kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH2O.
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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Mouse Umsila DNA Mini Kit Genomic DNA Extraction Kits ukusuka Mouse Umsila
Eyona khithi ikhawulezayo yehlabathi yokukhupha umsila wemouse i-genomic DNA, enokukhupha i-DNA ye-genomic ekumgangatho ophezulu kumsila wempuku kwiyure enye.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-Bacterial DNA Isolation Kit I-Bacterial Genomic DNA Extraction Iikhithi zokuCoca
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu evela kwiibhaktheriya kwisigaba sokukhula kwe-logarithmic.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-ATM/CSP11 iProbe yoMbala emibini
Ngokomgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, i-ATM e-orenji probe kunye ne-CSP11 eluhlaza isetyenziselwe ukuxutywa kunye nolandelelwano lwe-DNA ekujoliswe kuyo kwi-nucleus, kunye nolwazi lwesimo sofuzo kwi-nucleus yabonwa kwaye yahlalutywa phantsi kwe-microscope ye-fluorescence.
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ERG1/TERT iProbe yoMbala eMabini
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
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I-DNA/RNA Extraction Kit (Amaso kaMagnetic)
- Yonke inkqubo yokuzahlula ikhuselekile kwaye ifanelekile
- I-DNA ekhutshiweyo yamahhala inesivuno esiphezulu, ukucoceka okuphezulu kunye nomgangatho othembekileyo
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20q12/20q13.33 iProbe yoMbala emibini
Ngokomgaqo wokudityaniswa kokudityaniswa kweziseko ze-DNA, i-20q12 (D20S108) i-orenji probe kunye ne-20q33.3 ye-green probe isetyenziselwe ukuxubanisa ngolandelelwano lwethagethi ye-DNA kwinucleus, kunye nolwazi lwesimo sofuzo kwinucleus lwajongwa lwaza lwahlalutywa phantsi kwe-fluorescence microscope.
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p53/D13S319 iProbe yoMbala eMabini
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
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EndoFree Maxi Plasmid Kit (Spin Column)
Yonke inkqubo yokutsalwa ithatha iyure enye kuphela, iqinisekisa ukusebenza ngokufanelekileyo nangokukhawulezayo.
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MALT1 Dual Color Break Apart probe
Ngokomgaqo-siseko we-DNA yokudibanisa okuhambelanayo, i-MALT1 i-orenji-red probe kunye ne-MALT1 ye-green probe yayisetyenziselwa ukuxutywa kunye nolandelelwano olujoliswe kwi-DNA kwi-nucleus, kunye nolwazi lwe-gene state kwi-nucleus lujongwe kwaye luhlalutywe phantsi kwe-microscope ye-fluorescence.
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I-D7S522/CSP7 iProbe yoMbala emibini
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
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DAPI Counterstain
Inxalenye ephambili yesisombululo se-DAPI sokucoca kwakhona yi-4-phenylindole, enokungena kwi-membrane yeseli kwaye ibophe ngamandla kwi-DNA.
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