Ababoneleli be-reagent yeBhayoloji kunye neFactory |China Molecular Biology reagent Abavelisi-Icandelo 2

I-Reagent yeBhayoloji yeMolekyuli

Uluhlu lokuzihlukanisa lwe-RNA

DNA isolation series

Ngqo RT-qPCR series

Igazi ngqo PCR series

Tyala PCR ngqo series

Isilwanyana ngqo PCR series

PCR/RT-PCR series

IVD imathiriyeli ekrwada

Blood DNA Mini Kit

Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (<1ml).

◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.

◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.

◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.

◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.

◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.

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Ngokubanzi iPlasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits

Ucoca ngokukhawuleza i-plasmid DNA ekumgangatho ophezulu ukusuka kwiibhaktheriya eziguquliweyo zovavanyo lwesiqhelo lwebhayoloji yemolekyuli njengotshintsho kunye nokwetyisa kwe-enzyme.

◮Susa i-RNA ngaphandle kokongeza i-RNAse

◮Iluncedo-i-centrifugation yenziwa kwindawo yobushushu begumbi

◮Ukukhawuleza-umsebenzi unokugqitywa kwimizuzu engama-20

◮Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo

◮Ukucoceka okuphezulu—OD260/280≈1.7-1.9

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I-RT Easyᵀᴹ I Master Premix ye-cDNA yomtya wokuqala

- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-25 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.

-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.

-Inkqubo yokukhuphela umva inozinzo oluphezulu lwe-thermal, ubushushu bokusabela kwenkqubo bunokuphakama ukuya kuma-50 ℃,kwaye inomsebenzi olungileyo wokukhuphela umva.

- Nkqu neesampuli ze-RNA ezingcolileyo (utywala ukuya kwi-45%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.

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I-RT Easy II (kunye ne-gDNase) i-Master Premix yokuqala-strand ye-cDNA synthesis yeXesha lokwenyani PCR ene-gDNase

-Ikhono elisebenzayo lokususa i-gDNA, enokususa i-gDNA kwi-template ngaphakathi kwemizuzu emi-2.

-Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu eyi-15 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.

-Iitemplates eziyinkimbinkimbi: iitemplates ezinomxholo ophezulu weGC kunye nesakhiwo sesibini esiyinkimbinkimbi sinokuphinda sitshintshwe ngokusebenza okuphezulu.

-I-High-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.

I-reverse transcription system inozinzo oluphezulu lwe-thermal, iqondo lokushisa elichanekileyo liyi-42℃, kwaye isenomsebenzi olungileyo wokukhutshelwa umva kwi-50℃.

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Ixesha lokwenyani PCR Easyᵀᴹ-SYBR Green I kit

Lula—2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza

I-Specific-optimized buffer kunye ne-Hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.

Uvakalelo oluphezulu-luyakwazi ukubona iikopi eziphantsi zetemplate

Ukuguquguquka okuhle-kuyahambelana nezixhobo ezininzi zePCR zexesha lokwenyani

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ISilwanyana sisonke se-RNA Isolation Kit Iyonke yeRNA yokutsalwa kunye neeKits zokuCoca kwiiTissues zezilwanyana kunye neSeli

Ngokukhawuleza nangempumelelo ukukhupha ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu ezahlukeneyo zezilwanyana.

Akukho mfuneko yakukhathazeka malunga nokonakaliswa kwe-RNA.Yonke inkqubo i-RNase-Free

Susa ngokufanelekileyo i-DNA usebenzisa i-DNA-Cleaning Column

Susa i-DNA ngaphandle kokongeza i-DNase

Elula-yonke imisebenzi igqitywe kwiqondo lokushisa

Ukukhawuleza-ukusebenza kunokugqitywa kwimizuzu engama-30

Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo

Ukucoceka okuphezulu-OD260 / 280≈1.8-2.1

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ISeli iyonke yeRNA Isolation Kit Iyonke iRNA Isolation IiKits zokucoca ukusuka kwiseli

Isulungekiswe kakhulu kwaye ikumgangatho ophezulu we-RNA iyonke inokufumaneka kwiiseli ezahlukeneyo zenkcubeko kwimizuzu eli-11.

RNase-Free

Isebenza kubushushu begumbi (15-25℃)

Nge-DNA-Ukucoca iKholam

Isivuno esiphezulu se-RNA

Ukukhawuleza:Gqiba ukutsalwa ngemizuzu eyi-11

Ukhuseleko:Ayikho imichiza ye-Organic

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I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA kwiGazi

Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (1-5ml).

◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.

◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.

◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.

◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.

◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.

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Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction okanye Purifiction Kit from Buccal Swabs

Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwi-buccal swab / iisampulu zeKhadi le-FTA.

◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.

◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.

◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.

◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.

◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.

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Inkqubo yokuchonga ye-Foregene DNA 27Y

I-ForegeneI-DNA Identification System 27Y isebenzisa ubuchwepheshe bokulebula ngemibala emithandathu ye-fluorescent ukukhulisa i-27 Y-STR loci ngexesha elinye.Kubandakanywa: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS3, DY39, DY3F38, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ikiti inamandla okuthintela ukunqanda, kwaye inokukhulisa ngokuthe ngqo iphepha lokucoca, ikhadi le-FTA, i-cotton swab, i-saliva card, i-oral swab, kwaye ingasetyenziselwa ukukhupha kunye nokucoca i-templates ye-DNA kwizinto zecala.

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I-Foregene DNA Identification System 38Y-C (Case)

I-ForegeneInkqubo yokuchonga iDNA 38Y-UC usebenzisa itekhnoloji yokuleyibheli yemibala emithandathu yefluorescent ukukhulisa 38 Y chromosome STR loci , 3 Y-Indel locikwayeezimbini zolawulo lomgangatho wangaphakathi loci ngexesha elinyeukukhulisa Iisampulu zeCase ezithathwe kwiitemplates zeDNA ecocekileyo.

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I-Foregene DNA Identification System 30A (Ayikhutshiweyo)

Inkqubo yokuchonga ye-ForEGENE DNA 30A isebenzisa ubuchwepheshe bokulebula ngemibala emithandathu ye-fluorescent ukuzekhulisa 29autosomalSIndawo yeTR, NyeI-Amel locus kunye ne-Yindel locus enye ngexesha elinye, kwaye inokukhulisa ngokuthe ngqo iphepha lokucoca, i-FTAamakhadi, iintambo zomqhaphu, amakhadi amathe,kunye ne-mswab ngaphandle.

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