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Imbewu yeTyalo(i-Polysaccharide Polyphenol etyebileyo, encinci) I-PCR ethe ngqo kunye neKit I-UNG(ngaphandle kweZixhobo zokuSampula)
Sebenzisa ngokuthe ngqo i-polysaccharide polyphenol seed lysate njenge template yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
- Akukho ukuchitha ixesha kunye neendleko zokucocwa kweDNA.
- Imfuno yesampulu incinci, thatha nje imbewu enye.
- Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
-Isistim ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezela okunamandla kwi-PCR reaction inhibitors.
- Inkqubo ye-PCR yokuchasana nokungcoliswa kwe-2 × Imbewu ye-PCR Easy TM Mix (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.
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Imbewu yeTyalo (enkulu kunye nobukhulu obuphakathi) I-PCR ethe ngqo kunye neKit II-UNG(ngaphandle kweZixhobo zokuSampula)
Sebenzisa ngokuthe ngqo i-polysaccharide polyphenol seed lysate njenge template yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
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Imbewu yeTyalo(Polysaccharide Polyphenol rich Small) Direct PCR Plus Kit I (ngaphandle kweZixhobo zeSampulu)
Sebenzisa ngokuthe ngqo i-polysaccharide polyphenol seed lysate njenge template yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
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Imbewu yeTyalo(i-Polysaccharide Polyphenol etyebileyo, enkulu kunye nobukhulu obuphakathi) Direct PCR Plus Kit II-UNG(ngaphandle kweZixhobo zeSampulu)
Sebenzisa ngokuthe ngqo i-polysaccharide polyphenol seed lysate njenge template yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
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Imbewu yeTyalo(Enkulu) I-PCR Kit II-UNG ethe ngqo (ngaphandle kweZixhobo zokuSampula)
Ukusetyenziswa ngokuthe ngqo kwembewu ye-lysate njengethempleyithi yokukhulisa i-PCR, akukho kukhutshwa kwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
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I-ForeDirect RT-qPCR Kit
◮Kwi-RNA yokukhulisa ixesha lokwenyani ngqo ukusuka kwingqokelela ye-swab ngaphandle kweenkqubo zokucoca i-RNA.Le khithi igqiba imijikelo ye-qRT-PCR kwiyure enye.Umxube we-enzyme ngumxube ophuculweyo we-Reverse Transcriptase, i-Hot-Start Taq DNA polymerase, i-RNase inhibitor.I-Reaction Buffer iqulathe onke amacandelo afunekayo, kubandakanywa amacandelo esikhuseli esiphuculweyo, uMg2+, i-dUTP, kunye ne-dNTPs.
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I-PCR yokucoca iKhiti ye-PCR yokuCoca-up System ye-Ultra-pure DNA
Ngokukhawuleza uhlambulule kwaye ufumane amaqhekeza e-DNA akumgangatho ophezulu kwinkqubo yePCR;ukuba ufuna ukwahlula kwaye ufumane amaqhekeza athile eDNA, nceda ukhethe ikhithi yokubuyisela ijeli.
◮ Uluhlu olubanzi lokuchacha kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-60bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮ Ukusebenza okuphezulu kokubuyisela: Ukusebenza kokubuyisela ngokuqhelekileyo kungaphezulu kwe-80%, kwaye iphakamileyo inokufikelela ngaphezu kwe-95%.
◮ Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15 kwaye i-primer dimer inokususwa ngokuthe ngqo ngaphandle kokubuyiswa kwe-gel.
◮ Safety:Akukho kutsalwa kwe-organic reagent efunekayo.
◮ Umgangatho ophezulu: Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana novavanyo olwahlukeneyo olulandelayo.
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I-Stool DNA Isolation Kit DNA Extraction Iikhithi zokucoca ukusuka kwisitulo
Ucoca ngokukhawuleza kwaye ufumane i-DNA ye-genomic ekumgangatho ophezulu kwiisampulu zesitulo ezahlukahlukeneyo.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Iarhente yokukhupha iSampuli
◮Kulula ukusebenza:isampuli lysis ngelixa ingqokelela isampuli
◮Khawuleza kwaye lufanelekile:Akukho mfuneko yokutsalwa kwe-nucleic acid, ilungele ukuchongwa kwesikali esikhulu
◮ Umda obanzi wesicelo:I-PCR ngokuthe ngqo emva kokuqokelela isampulu
◮Ikhuselekile kwaye ithembekile:Yenza intsholongwane ngokukhawuleza ingasebenzi, qinisekisa ukhuseleko ukuya kuthi ga kwinqanaba elikhulu
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I-Soil DNA Isolation Kit yeKhitshi yokuNxila iiKhiti zokuCoca & ii-reagents ze-DNA yomhlaba
Ngokukhawuleza ucoce kwaye ufumane i-DNA ye-genomic ekumgangatho ophezulu kwiisampulu zesampulu zomhlaba ezahlukeneyo.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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FFPE DNA Isolation Kit DNA Extraction Ikhithi yokuCoca kwiiSampuli zeFFPE
Ngokukhawuleza coca i-DNA ye-genomic ekumgangatho ophezulu ukusuka kwizihlunu ezifakwe kwiparafini ezifana namacandelo eparafini kunye neebhloko zeparafini.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-RT-PCR Easyᵀᴹ II(Amanyathelo amabini)
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮ Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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