- Nye-Ikiti yenyathelo yenza ukuba uguqulelo oluguqulelwe kunye ne-PCR luqhutywe kwityhubhu enye, kufuneka kuphela ukongeza i-template ye-RNA, iiprimers ezithile ze-PCR kunye ne-RNase-Free ddH.2O.
- Uhlalutyo lwexesha langempela lobungakanani bentsholongwane yeRNA okanye umkhondo weRNA unokuqhutywa ngokukhawuleza nangokuchanekileyo.
- Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
-Inkqubo yokusabela eyongeziweyo yenza ukusabela kube novakalelo oluphezulu lokubona, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
- RT-qPCR EasyTM(Inyathelo elinye) -Ikhithi yeTaqman iza ne-ROX yedayi yesalathiso yangaphakathi, enokusetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesiginali phakathi kwamaqula, elungele ukusetyenziswa kwabasebenzisi bokugqibela kwiimodeli ezahlukeneyo zezixhobo zePCR zobungakanani.